National Key Laboratory of Plant Molecular Genetics, CAS Center for Excellence in Molecular Plant Sciences, Institute of Plant Physiology and Ecology, Chinese Academy of Sciences; University of Chinese Academy of Sciences, Shanghai 200032, China.
National Key Laboratory of Crop Genetic Improvement, Hubei Key Laboratory of Agricultural Bioinformatics, Hubei Engineering Technology Research Center of Agricultural Big Data, College of Informatics, Huazhong Agricultural University, Wuhan 430070, China.
Nucleic Acids Res. 2019 Sep 5;47(15):7857-7869. doi: 10.1093/nar/gkz511.
Autopolyploidy is widespread in higher plants and important for agricultural yield and quality. However, the effects of genome duplication on the chromatin organization and transcriptional regulation are largely unknown in plants. Using High-throughput Chromosome Conformation Capture (Hi-C), we showed that autotetraploid Arabidopsis presented more inter-chromosomal interactions and fewer short-range chromatin interactions compared with its diploid progenitor. In addition, genome duplication contributed to the switching of some loose and compact structure domains with altered H3K4me3 and H3K27me3 histone modification status. 539 genes were identified with altered transcriptions and chromatin interactions in autotetraploid Arabidopsis. Especially, we found that genome duplication changed chromatin looping and H3K27me3 histone modification in Flowering Locus C. We propose that genome doubling modulates the transcription genome-wide by changed chromatin interactions and at the specific locus by altered chromatin loops and histone modifications.
自体多倍体在高等植物中广泛存在，对农业产量和质量很重要。然而，在植物中，基因组加倍对染色质组织和转录调控的影响在很大程度上是未知的。我们使用高通量染色体构象捕获（Hi-C）技术，发现与二倍体祖先相比，自体四倍体拟南芥呈现出更多的染色体间相互作用，而短程染色质相互作用较少。此外，基因组加倍导致一些松散和紧密结构域的转换，其 H3K4me3 和 H3K27me3 组蛋白修饰状态发生改变。在自体四倍体拟南芥中，有 539 个基因的转录和染色质相互作用发生了改变。特别是，我们发现基因组加倍改变了花分生组织中心的染色质环和 H3K27me3 组蛋白修饰。我们提出，基因组加倍通过改变染色质相互作用，在特定基因座通过改变染色质环和组蛋白修饰，来广泛调节转录组。
