Department of Molecular Diagnosis Center, The Sixth Affiliated Hospital of Guangzhou Medical University, Qingyuan People's Hospital, No. B24, Xincheng Yinquan Road, Qingcheng District, Qingyuan, 511518, Guangdong, China.
Otorhinolaryngology, The Sixth Affiliated Hospital of Guangzhou Medical University, Qingyuan People's Hospital, Guangdong, 511518, Qingyuan, China.
Virol J. 2020 Oct 16;17(1):155. doi: 10.1186/s12985-020-01423-w.
This study aims to screen the male human papillomavirus (HPV) infection status and genotyping in Qingcheng District, Qingyuan City, Guangdong Province, China to provide a reference basis for formulating prevention strategies for HPV infection.
The present study collected urethral epithelium or scraped penile epidermis from high-risk male patients in Qingyuan People's Hospital during the last five years, extracted DNA fragments using the boiling method, and detected 23 types of HPV genotypes by PCR-reverse blot hybridization.
The positive detection rate was 54.31% of 1044 males with high risk of HPV (567/1044). Among these males, the positive detection rate of HPV was the highest in patients initially diagnosed with warts, and the rate was 66.47%. Five main HPV types are identified as follows: HPV6 18.87% (197/1044), HPV11 10.25% (107/1044), HPV52 8.81% (92/1044), HPV16 6.90% (72/1044), and HPV51 5.08% (53/1044). Among these HPV-infected patients, single infection mainly by low-risk HPV6 and HPV11 accounted for 56.61% (321/567); high- and low-risk combined HPV co-infections accounted for 29.10% (165/567). The HPV infected patients was mainly between 21 and 40 years old, and the HPV infection rate was higher with increased age.
The HPV infection rate in the Qingyuan area is higher than in other areas and the main infection is single infection. Furthermore, HPV52, HPV16, and HPV51 are the main high-risk infection types, while HPV6 and HPV11 are the main low-risk infection types.
本研究旨在筛查中国广东省清远市清城区男性人乳头瘤病毒（HPV）感染状况和基因型，为制定 HPV 感染防控策略提供参考依据。
本研究收集清远市人民医院高危男性患者五年间的尿道上皮或阴茎表皮刮取物，采用煮沸法提取 DNA 片段，采用 PCR-反向斑点杂交法检测 23 种 HPV 基因型。
1044 例高危 HPV 男性患者中，阳性检出率为 54.31%（567/1044）。其中初诊为疣的患者 HPV 阳性检出率最高，为 66.47%。五种主要 HPV 类型分别为：HPV6 18.87%（197/1044）、HPV11 10.25%（107/1044）、HPV52 8.81%（92/1044）、HPV16 6.90%（72/1044）、HPV51 5.08%（53/1044）。在这些 HPV 感染患者中，单一感染以低危 HPV6 和 HPV11 为主，占 56.61%（321/567）；高低危混合 HPV 共感染占 29.10%（165/567）。HPV 感染患者主要集中在 21~40 岁，随年龄增长 HPV 感染率升高。
清远地区 HPV 感染率高于其他地区，以单一感染为主。HPV52、HPV16 和 HPV51 为主要高危感染型，HPV6 和 HPV11 为主要低危感染型。
