Nishi Yasunobu, Tsukano Kenji, Otsuka Marina, Tsuchiya Masakazu, Suzuki Kazuyuki
School of Veterinary Medicine, Rakuno Gakuen University, 582 Bunkyodai-Midorimachi, Ebetsu, Hokkaido 069-8501, Japan.
Microbial Solutions, Charles River, 1023 Wappoo Road, Suite 43B, Charleston, SC 29407, U.S.A.
J Vet Med Sci. 2019 Jul 19;81(7):1043-1046. doi: 10.1292/jvms.18-0643. Epub 2019 Jun 12.
The aim of this study was to investigate the relationship between the endotoxin activity in plasma and that in bronchoalveolar lavage fluid (BALF) in bronchopneumonia. Thirty-three calves were included in this study (17 healthy calves and 16 calves with respiratory disease). In the calves with bronchopneumonia, the median endotoxin activity in plasma (0.437 EU/ml, P<0.001) and BALF (29.45 EU/ml, P<0.001) was significantly higher than in the control calves. Plasma endotoxin activity was significantly and positively correlated with that in BALF (r=0.900, P<0.001). Based on the receiver operating characteristics curves, we propose a diagnostic cutoff point for plasma endotoxin activity (0.104 EU/ml, AUC=0.914, P<0.001, Se 81.3% and Sp 82.4%) for identification of bronchopneumonia in calves which could die within a week.
本研究的目的是调查支气管肺炎中血浆内毒素活性与支气管肺泡灌洗液（BALF）内毒素活性之间的关系。本研究纳入了33头犊牛（17头健康犊牛和16头患有呼吸系统疾病的犊牛）。患有支气管肺炎的犊牛血浆内毒素活性中位数（0.437 EU/ml，P<0.001）和BALF内毒素活性中位数（29.45 EU/ml，P<0.001）显著高于对照犊牛。血浆内毒素活性与BALF内毒素活性显著正相关（r=0.900，P<0.001）。基于受试者工作特征曲线，我们提出了一个血浆内毒素活性的诊断临界值（0.104 EU/ml，AUC=0.914，P<0.001，敏感性81.3%，特异性82.4%），用于识别一周内可能死亡的犊牛支气管肺炎。
