评价从（L.）poit 中分离得到的提取物和化合物对乳腺癌细胞的细胞毒性。

Evaluation of the cytotoxicity on breast cancer cell of extracts and compounds isolated from (L.) poit.

机构信息

Departamento de Farmácia, Universidade Federal de Sergipe, São Cristóvão, SE, Brasil.

Departamento de Gerontologia, Universidade Federal de São Carlos, São Carlos, SP, Brasil.

出版信息

Nat Prod Res. 2020 Jan;34(1):102-109. doi: 10.1080/14786419.2019.1628747. Epub 2019 Jun 13.

DOI:10.1080/14786419.2019.1628747

PMID:31192696

Abstract

is a herb popularly used in Brazil for the treatment of inflammations, pain, bacterial infections and cancer. In the present study, inflorescences (MPIn), leaves (MPL), branches (MPB), root (MPR) extracts and three compounds isolated from MPIn were assayed against breast tumor cell lines. The structures of the three compounds (pectinolide J, hyptolide and pectinolide E) were determined by means of spectroscopic analysis. Pectinolide J was isolated for the first time. The MPIn, MPL and MPR exhibited specific antiproliferative activity on tumor cell lines when compared to normal cell lines with IC of 52.01 ± 0.64, 45.91 ± 0.02 μg/mL and 82.84 ± 0.03 μg/mL, respectively. Although the isolated substances did not present good antiproliferative activity, when the three were associated, a greater biological effect was observed, suggesting a synergistic effect. Hyptolide (5.6 ± 0.4 μg/mL) showed IC sufficiently low to be considered as a drug prototype.

摘要

是一种在巴西广泛用于治疗炎症、疼痛、细菌感染和癌症的草药。在本研究中，对花序（MPIn）、叶（MPL）、枝（MPB）、根（MPR）提取物和从 MPIn 中分离的三种化合物进行了抗乳腺癌细胞系的测定。三种化合物（pectinolide J、hyptolide 和 pectinolide E）的结构通过光谱分析确定。pectinolide J 是首次分离得到的。与正常细胞系相比，MPIn、MPL 和 MPR 对肿瘤细胞系具有特定的抗增殖活性，IC 分别为 52.01±0.64、45.91±0.02 和 82.84±0.03μg/mL。尽管分离出的物质没有表现出良好的抗增殖活性，但当这三种物质联合使用时，观察到更大的生物学效应，表明存在协同作用。Hyptolide（5.6±0.4μg/mL）的 IC 足够低，可以被认为是药物原型。