The Education Ministry Key Lab of Resource Chemistry and Shanghai Key Laboratory of Rare Earth Functional Materials, Shanghai Normal University, Shanghai, 200234, People's Republic of China; Institute of Environmental and Operational Medicine, Academy of Military Medical Sciences, Academy of Military Sciences, Tianjin, 300050, People's Republic of China.
Institute of Environmental and Operational Medicine, Academy of Military Medical Sciences, Academy of Military Sciences, Tianjin, 300050, People's Republic of China.
Anal Chim Acta. 2023 Aug 1;1267:341351. doi: 10.1016/j.aca.2023.341351. Epub 2023 May 10.
Food safety is one of the greatest public health challenges. Developing ultrasensitive detection methods for analytes at ultra-trace levels is, therefore, essential. In recent years, the bio-barcode assay (BCA) has emerged as an effective ultrasensitive detection strategy that is based on the indirect amplification of various DNA probes. This review systematically summarizes the progress of fluorescence, PCR, and colorimetry-based BCA methods for the detection of various contaminants, including pathogenic bacteria, toxins, pesticides, antibiotics, and other chemical substances in food in over 120 research papers. Current challenges, including long experimental times and strict storage conditions, and the prospects for the application of BCA in biomedicine and environmental analyses, have also been discussed herein.
食品安全是最大的公共卫生挑战之一。因此，开发用于痕量分析物的超灵敏检测方法至关重要。近年来，生物条码分析（BCA）作为一种有效的超灵敏检测策略已经出现，它基于各种 DNA 探针的间接扩增。本综述系统地总结了基于荧光、PCR 和比色法的 BCA 方法在检测食品中各种污染物（包括致病菌、毒素、农药、抗生素和其他化学物质）方面的进展，共涉及 120 多篇研究论文。本文还讨论了当前面临的挑战，包括实验时间长、储存条件严格，以及 BCA 在生物医学和环境分析中的应用前景。
