Department of Neurology and Suzhou Clinical Research Center of Neurological Disease, The Second Affiliated Hospital of Soochow University, Suzhou 215004, China; Jiangsu Key Laboratory of Neuropsychiatric Diseases and Institute of Neuroscience, Soochow University, Suzhou, Jiangsu 215123, China.
Department of Neurology and Suzhou Clinical Research Center of Neurological Disease, The Second Affiliated Hospital of Soochow University, Suzhou 215004, China.
Behav Brain Res. 2019 Oct 17;372:112031. doi: 10.1016/j.bbr.2019.112031. Epub 2019 Jun 10.
Parkinson's disease (PD) is the second most common neurodegenerative disorder in the world. Apart from motor deficits, PD reduces patient's quality of life through sleep disturbances, cognitive impairment and emotional disorders. However, it's unclear whether bad life habits such as stay up late exacerbate the patient's cognition and emotional disorders. Thus we investigated the consequences of sleep deprivation (SD) on memory and emotions using a rotenone-based zebrafish model of PD. Behavioral assays, using locomotor activity assay, showed that rotenone treated zebrafish exhibited PD-like symptoms, whereas sleep deprivation didn't exacerbate the progression of them. The object discrimination task exhibited that the short-term cognitive deficits of rotenone group are more serious than the sham group after SD. Light-dark box test showed that rotenone treated fish are more dysphoric than the sham fish after SD. Dopamine and DOPAC significantly reduced in rotenone treated fish compared with the sham fish. However, this DOPAC reduction recovered after SD. The expression of D2 and D3 in rotenone treated zebrafish elevated compared with sham group and SD group. However, the rotenone treated zebrafish manifested a decrease level of D2 and D3 after SD. D1 did not show any significantly changes among the four groups. Our findings suggest that zebrafish treated with rotenone may have a more severe damage of memory and emotional function after SD, which may be related to the changes in the DA systems.
帕金森病（PD）是世界上第二常见的神经退行性疾病。除了运动缺陷外，PD 通过睡眠障碍、认知障碍和情绪障碍降低了患者的生活质量。然而，目前尚不清楚熬夜等不良生活习惯是否会加重患者的认知和情绪障碍。因此，我们使用基于鱼藤酮的 PD 斑马鱼模型研究了睡眠剥夺（SD）对记忆和情绪的影响。行为学分析，使用运动活性测定法，表明鱼藤酮处理的斑马鱼表现出 PD 样症状，而睡眠剥夺并没有加剧它们的进展。物体识别任务表明，SD 后，鱼藤酮组的短期认知缺陷比假手术组更严重。明暗箱测试表明，SD 后，鱼藤酮处理的鱼比假手术组的鱼更烦躁。与假手术组相比，鱼藤酮处理的鱼中的多巴胺和 DOPAC 显著减少。然而，这种 DOPAC 减少在 SD 后得到恢复。与假手术组和 SD 组相比，鱼藤酮处理的斑马鱼中 D2 和 D3 的表达升高。然而，SD 后，鱼藤酮处理的斑马鱼的 D2 和 D3 水平下降。D1 在四组之间没有显示出任何显著变化。我们的研究结果表明，鱼藤酮处理的斑马鱼在 SD 后可能会出现更严重的记忆和情绪功能损伤，这可能与 DA 系统的变化有关。
