Pang Meng-Zhu, Li Han-Xing, Dai Xue-Qin, Wang Xiao-Bo, Liu Jun-Yi, Shen Yun, Xu Xing, Zhong Zhao-Min, Wang Han, Liu Chun-Feng, Wang Fen
Department of Neurology and Clinical Research Center of Neurological Disease, The Second Affiliated Hospital of Soochow University, Suzhou, 215004, China.
Jiangsu Key Laboratory of Neuropsychiatric Diseases and Institutes of Neuroscience, Soochow University, Suzhou, 215123, China.
Neurosci Bull. 2024 Dec;40(12):1901-1914. doi: 10.1007/s12264-024-01299-8. Epub 2024 Sep 16.
Sleep-wake disorder is one of the most common nonmotor symptoms of Parkinson's disease (PD). Melatonin has the potential to improve sleep-wake disorder, but its mechanism of action is still unclear. Our data showed that melatonin only improved the motor and sleep-wake behavior of a zebrafish PD model when melatonin receptor 1 was present. Thus, we explored the underlying mechanisms by applying a rotenone model. After the PD zebrafish model was induced by 10 nmol/L rotenone, the motor and sleep-wake behavior were assessed. In situ hybridization and real-time quantitative PCR were used to detect the expression of melatonin receptors and lipid-metabolism-related genes. In the PD model, we found abnormal lipid metabolism, which was reversed by melatonin. This may be one of the main pathways for improving PD sleep-wake disorder.
睡眠-觉醒障碍是帕金森病（PD）最常见的非运动症状之一。褪黑素具有改善睡眠-觉醒障碍的潜力，但其作用机制仍不清楚。我们的数据表明，只有当存在褪黑素受体1时，褪黑素才能改善斑马鱼PD模型的运动和睡眠-觉醒行为。因此，我们通过应用鱼藤酮模型探索其潜在机制。用10 nmol/L鱼藤酮诱导建立PD斑马鱼模型后，评估其运动和睡眠-觉醒行为。采用原位杂交和实时定量PCR检测褪黑素受体和脂质代谢相关基因的表达。在PD模型中，我们发现脂质代谢异常，而褪黑素可使其逆转。这可能是改善PD睡眠-觉醒障碍的主要途径之一。
