Nanotechnology Centre, VŠB - Technical University of Ostrava, 17. listopadu 15/2172, Ostrava 708 33, Czech Republic.
J Nanosci Nanotechnol. 2019 Dec 1;19(12):7926-7933. doi: 10.1166/jnn.2019.15854.
Bionanotechnology provides many new methods and protocols in nanomaterial preparation. One of these special new chemical methods is phytosynthesis. The application of biological processes in living organisms such as bacteria, fungi and higher plants contributes to rapid and easy formation of metallic nanoparticles. These nanoparticles present a promising future in heterogeneous catalysis and medicine, and here we focus on phytosynthesis of Ag, ZnO and ZrO₂ nanoparticles using leachate from the linden plant. Nanoparticle activity in liquid is an important aspect of their behavior, and we investigated nanoparticles -potential and monitored their particle size by dynamic light scattering during the period of three months. Transmission electron microscopy then determined shape and morphology, with results confirming their spherical shape and average size in tens and hundreds of nanometers. The amount of metals was estimated in tens of mg L and the different nanoparticle sizes obtained by dynamic light scattering and transmission electron microscopy are discussed. The nanoparticles were tested against 4 human pathogens using minimum inhibitory concentration to investigate their antimicrobial potential. Only Ag nanoparticles provided antibacterial properties against ; with the remaining nanoparticles having no antibacterial effect on the four tested pathogens. All studied phenomena are related to nanoparticle concentration and their surface charge, and therefore -potential and other physical and chemical properties are important in ascertaining positive and negative aspects of metallic nanoparticles in future applications and related research.
生物纳米技术在纳米材料的制备方面提供了许多新的方法和方案。这些特殊的新化学方法之一是植物合成法。在细菌、真菌和高等植物等生物体中应用生物过程有助于快速而容易地形成金属纳米粒子。这些纳米粒子在多相催化和医学方面具有广阔的应用前景，在此我们集中研究了利用菩提树浸出液来合成 Ag、ZnO 和 ZrO₂纳米粒子。纳米粒子在液体中的活性是其行为的一个重要方面，我们通过动态光散射法在三个月的时间内研究了纳米粒子的 -电位，并监测了它们的粒径。然后通过透射电子显微镜确定了它们的形状和形态，结果证实了它们的球形和数十到数百纳米的平均粒径。通过动态光散射和透射电子显微镜获得的不同纳米粒子尺寸与数十毫克/升的金属量相对应。研究了这些纳米粒子对 4 种人类病原体的最小抑菌浓度，以研究它们的抗菌潜力。只有 Ag 纳米粒子对表现出抗菌特性，而其余的纳米粒子对这 4 种测试的病原体没有抗菌作用。所有研究的现象都与纳米粒子的浓度及其表面电荷有关，因此 -电位和其他物理化学性质在确定金属纳米粒子在未来应用和相关研究中的积极和消极方面非常重要。
