Department of Dermatology, Suleyman Demirel University Faculty of Medicine, Isparta, Turkey.
Department of Medical Biochemistry, Suleyman Demirel University Faculty of Medicine, Isparta, Turkey.
J Eur Acad Dermatol Venereol. 2019 Oct;33(10):1952-1957. doi: 10.1111/jdv.15746. Epub 2019 Jul 3.
BACKGROUND: Warts are benign conditions of the skin and mucosa caused by human papilloma viruses (HPV) that affect many people worldwide. OBJECTIVE: The aim of this study was to evaluate OS by TOS/TAS, levels of 8-hydroxy-2-deoxyguanosine (8-OHdG) an indicator of DNA damage, and also protein oxidation levels by determining the dynamic serum thiol/disulphide homeostasis in patients with warts. We also aimed to investigate whether there is a relationship between thiol/disulphide homeostasis, recalcitrance of warts and DNA damage. METHODS: Forty patients of age ≥18 years, having recalcitrant genital and/or non-genital warts that persisted for more than 2 years, 40 patients with warts that persisted for <2 years and 40 healthy controls were enrolled in the study. Blood TAS, TOS, OSI, 8-OHdG and dynamic thiol/disulphide homeostasis were evaluated. RESULTS: Significant differences were detected between the groups in the levels of 8-OHdG, TOS, OSI, total thiol, native thiol, reduced thiol, as well as native thiol/total thiol ratio, disulphide/total thiol ratio and disulphide/native thiol ratio. Compared with the controls, patients with recalcitrant warts had significantly higher levels of 8-OHdG, TOS and OSI levels. Total thiol and native thiol levels were significantly lower in patients with recalcitrant warts compared with patients with warts that persisted for <2 years. Disulphide levels were significantly higher in the latter group of patients compared with patients with recalcitrant warts and controls. Native thiol/total thiol ratio was significantly higher in both patient groups compared with controls whereas disulphide/total thiol and disulphide/native thiol ratios were significantly lower in both patient groups than in controls. CONCLUSION: Our findings suggest that impairment of thiol disulphide homeostasis in patients with recalcitrant warts may lead to increased OS and DNA damage. Thus, antioxidant administration with thiol containing proteins may help in the regression of warts and thereby prevent carcinogenesis.
背景：疣是由人乳头瘤病毒（HPV）引起的皮肤和黏膜良性病变，影响着全世界许多人。
目的：本研究旨在评估 OS 通过 TOS/TAS、8-羟基-2-脱氧鸟苷（8-OHdG）的水平（一种 DNA 损伤的指标），以及通过确定动态血清巯基/二硫键动态平衡来评估患者疣中的蛋白质氧化水平。我们还旨在研究巯基/二硫键动态平衡与疣的难治性和 DNA 损伤之间是否存在关系。
方法：纳入了年龄≥18 岁、患有难治性生殖器和/或非生殖器疣且持续>2 年、患有持续<2 年的疣和 40 名健康对照者的 40 名患者。评估了血液 TAS、TOS、OSI、8-OHdG 和动态巯基/二硫键动态平衡。
结果：在 8-OHdG、TOS、OSI、总巯基、天然巯基、还原巯基以及天然巯基/总巯基比值、二硫键/总巯基比值和二硫键/天然巯基比值方面，各组之间存在显著差异。与对照组相比，难治性疣患者的 8-OHdG、TOS 和 OSI 水平显著升高。与持续<2 年的疣患者相比，难治性疣患者的总巯基和天然巯基水平显著降低。与难治性疣患者和对照组相比，后者组的二硫键水平显著升高。与对照组相比，两组患者的天然巯基/总巯基比值均显著升高，而两组患者的二硫键/总巯基比值和二硫键/天然巯基比值均显著降低。
结论：我们的研究结果表明，难治性疣患者巯基二硫键动态平衡受损可能导致 OS 和 DNA 损伤增加。因此，含巯基蛋白的抗氧化剂给药可能有助于疣的消退，从而预防癌变。
J Eur Acad Dermatol Venereol. 2019-7-3
Medicina (Kaunas). 2019-6-14
Niger J Clin Pract. 2020-10
Eur J Trauma Emerg Surg. 2019-2
Biomolecules. 2024-9-18
Life (Basel). 2023-6-23
J Clin Med. 2022-3-9
Zotero 插件