Department of Physiology, Medical Faculty, Harran University, 63000 Sanliurfa, Turkey.
Department of Endocrinology, Medical Faculty, Harran University, 63000 Sanliurfa, Turkey.
Medicina (Kaunas). 2019 Jun 14;55(6):275. doi: 10.3390/medicina55060275.
: The aim of this study was to research oxidative stress and thiol/disulphide homeostasis in Graves' patients. : The study included 33 Graves' patients (research group) and 35 healthy subjects (control group). Serum oxidative stress and thiol/disulphide homeostasis (a new and automated spectrophotometric method developed by Erel and Neselioglu) parameters were studied and compared between the groups. The native and total thiol levels and the native thiol/total thiol ratio were lower in patients with Graves' disease compared to the control group ( < 0.001, < 0.001, and = 0.006, respectively). TOS (total antioxidant status), PC (protein carbonyl), OSI (Oxidative stress index), and disulphide/native thiol and disulphide/total thiol ratios were determined to be higher in the Graves' disease group than in the control group ( < 0.001, = 0.001, = 0.001, = 0.004, and = 0.006, respectively). In the Graves' disease group, the free triiodothyronine (FT3) and free thyroxine (FT4) levels were significantly positively correlated with impaired thiol/disulphide homeostasis and oxidative stress parameters ( < 0.05). : The results of the current study demonstrated that oxidative stress and thiol/disulphide homeostasis increased towards disulphide formation due to thiol oxidation in Graves' disease. In addition, a positive correlation of FT3 and FT4 was observed with oxidative stress parameters and impaired thiol/disulphide homeostasis.
: 本研究旨在研究格雷夫斯病患者的氧化应激和硫醇/二硫键动态平衡。 : 该研究纳入了 33 例格雷夫斯病患者（研究组）和 35 名健康受试者（对照组）。研究比较了两组患者的血清氧化应激和硫醇/二硫键动态平衡（Erel 和 Neselioglu 开发的一种新的自动化分光光度法）参数。与对照组相比，格雷夫斯病患者的天然和总巯基水平以及天然巯基/总巯基比值较低（<0.001，<0.001 和=0.006）。与对照组相比，TOS（总抗氧化状态）、PC（蛋白羰基）、OSI（氧化应激指数）以及二硫键/天然巯基和二硫键/总巯基比值在格雷夫斯病组更高（<0.001，=0.001，=0.001，=0.004 和=0.006）。在格雷夫斯病组中，游离三碘甲状腺原氨酸（FT3）和游离甲状腺素（FT4）水平与巯基/二硫键动态平衡和氧化应激参数受损呈显著正相关（<0.05）。 : 本研究结果表明，由于巯基氧化，格雷夫斯病患者的氧化应激和硫醇/二硫键动态平衡向二硫键形成方向增强。此外，FT3 和 FT4 与氧化应激参数和巯基/二硫键动态平衡受损呈正相关。
