伯氏疏螺旋体向蜱蛋白 Salp12 的趋化作用有助于获得。
Borrelia burgdorferi chemotaxis toward tick protein Salp12 contributes to acquisition.
机构信息
Section of Infectious Diseases, Department of Internal Medicine, Yale University School of Medicine, New Haven, CT 06519, USA.
Section of Infectious Diseases, Department of Internal Medicine, Yale University School of Medicine, New Haven, CT 06519, USA; Center for AIDS Research, Laboratory of Biochemical Pharmacology, Department of Pediatrics, Emory University School of Medicine, Atlanta, GA 30322, USA.
出版信息
Ticks Tick Borne Dis. 2019 Aug;10(5):1124-1134. doi: 10.1016/j.ttbdis.2019.06.002. Epub 2019 Jun 8.
Lyme disease is a common tick-borne infection caused by the spirochete Borrelia burgdorferi sensu stricto (s.s.). B. burgdorferi s.s. may utilize chemotaxis, the directional migration towards or away from a chemical stimulus, for transmission, acquisition, and infection. However, the specific signals recognized by the spirochete for these events have not been defined. In this study, we identify an Ixodes scapularis salivary gland protein, Salp12, that is a chemoattractant for the spirochete. We demonstrate that Salp12 is expressed in the I. scapularis salivary glands and midgut and expression is not impacted by B. burgdorferi s.s. infection. Knockdown of Salp12 in the salivary glands or passive immunization against Salp12 reduces acquisition of the spirochete by ticks but acquisition is not completely prevented. Knockdown does not impact transmission of B. burgdorferi s.s. This work suggests a new role for chemotaxis in acquisition of the spirochete and suggests that recognition of Salp12 contributes to this phenomenon.
莱姆病是一种常见的蜱传感染病，由伯氏疏螺旋体严格种（s.s.）引起。伯氏疏螺旋体 s.s. 可能利用趋化性，即朝向或远离化学刺激物的定向迁移，来进行传播、获取和感染。然而，对于这些事件，螺旋体识别的特定信号尚未确定。在这项研究中，我们鉴定出一种伊氏扇头蜱唾液腺蛋白 Salp12，它是螺旋体的趋化引诱剂。我们证明 Salp12 在伊氏扇头蜱的唾液腺和中肠中表达，并且表达不受伯氏疏螺旋体 s.s. 感染的影响。Salp12 在唾液腺中的敲低或针对 Salp12 的被动免疫会降低蜱对螺旋体的获取，但并不能完全阻止获取。敲低不会影响伯氏疏螺旋体 s.s. 的传播。这项工作表明趋化性在螺旋体的获取中发挥了新的作用，并表明对 Salp12 的识别有助于这一现象。
Zotero 插件