中华畲族群体中金眼™ DNA ID 22NC试剂盒21个常染色体STR基因座的遗传特征分析
Genetic characterization of 21 autosomal STR loci of Goldeneye™ DNA ID 22NC Kit in Chinese She group.
作者信息
Zhang Jingyi, Yang Zihao, Zhang Xiaochun, Tao Ruiyang, Zhang Jiashuo, Chen Chong, Zhang Suhua, Li Chengtao
机构信息
Department of Forensic Science, Medical School of Soochow University, Suzhou 215123, PR China; Shanghai Key Laboratory of Forensic Medicine, Shanghai Forensic Service Platform, Academy of Forensic Sciences, Ministry of Justice, Shanghai 200063, PR China.
Shanghai Key Laboratory of Forensic Medicine, Shanghai Forensic Service Platform, Academy of Forensic Sciences, Ministry of Justice, Shanghai 200063, PR China; Department of Forensic Medicine, School of Basic Medical Science, Wenzhou Medical University, Wenzhou 325035, PR China.
出版信息
Leg Med (Tokyo). 2019 Jul;39:45-48. doi: 10.1016/j.legalmed.2019.06.005. Epub 2019 Jun 11.
Genetic polymorphism of 21 autosomal STR loci included in Goldeneye™ DNA ID 22NC Kit (D19S253, D6S477, D22GATA198B05, D15S659, D8S1132, D3S1358, D3S3045, D14S608, D17S1290, D3S1744, D2S411, D18S535, D13S325, D7S1517, D10S1435, D11S2368, D4S2366, D1S1656, D7S3048, D10S1248 and D5S2500) was studied in 297 unrelated She individuals. Allele frequencies and forensic efficiency parameters such as, observed and expected heterozygosity (H & H), matching probability (MP), power of discrimination (PD), polymorphism information content (PIC), power of exclusion (PE), typical paternity index (TPI) was calculated for the loci. The combined PD and PE for all 21 STR loci were calculated to be 0.999999999999999999999999324094 and 0.999999996182425, respectively. The dataset indicated the usefulness of these loci in personal identification, parentage testing and complex kinship analysis in She population. The MDS plots and neighbor-joining tree were constructed based on pair-wise Nei's genetic distance by comparing allele frequency data for the 21 loci with ten other populations. The analysis showed that She population lies closer to a clade consisting Southern Han, Hainan Han and Li populations.
在297名无血缘关系的畲族个体中，研究了包含在Goldeneye™ DNA ID 22NC试剂盒中的21个常染色体STR基因座（D19S253、D6S477、D22GATA198B05、D15S659、D8S1132、D3S1358、D3S3045、D14S608、D17S1290、D3S1744、D2S411、D18S535、D13S325、D7S1517、D10S1435、D11S2368、D4S2366、D1S1656、D7S3048、D10S1248和D5S2500）的基因多态性。计算了这些基因座的等位基因频率和法医效率参数，如观察到的和预期的杂合度（H&H）、匹配概率（MP）、鉴别力（PD）、多态性信息含量（PIC）、排除力（PE）、典型父权指数（TPI）。计算得出所有21个STR基因座的组合PD和PE分别为0.999999999999999999999999324094和0.999999996182425。该数据集表明这些基因座在畲族人群的个人识别、亲子鉴定和复杂亲缘关系分析中有用。通过将21个基因座的等位基因频率数据与其他十个群体进行比较，基于成对的Nei遗传距离构建了MDS图和邻接树。分析表明，畲族人群更接近由南方汉族、海南汉族和黎族组成的一个分支。
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