Department of Chemical, Aerosol and Biological Hazards, Central Institute for Labour Protection - National Research Institute, Czerniakowska 16, 00-701, Warsaw, Poland.
Environ Monit Assess. 2019 Jun 17;191(7):444. doi: 10.1007/s10661-019-7568-z.
Pararosaniline hydrochloride (CPR) is a dye used for colouring paper, leather and natural and artificial fibres. It is also used in analytical and microbiological laboratories. It is a carcinogenic substance of category 1B. In analytical chemistry, it is used for detecting the following among others: bromates, formaldehyde, ozone, sulphite and sulfur dioxide. CPR is a dye commonly used in microbiology for staining preparations, for staining bacteria, antibodies or other organisms. In Poland, about 800 employees were exposed to this substance. The lack of methods for the determination of pararosaniline hydrochloride in workplace air makes it impossible to assess the occupational exposure of workers to this substance. For this reason, a determination method has been developed, which allows for the determination of pararosaniline hydrochloride in the air. This method makes it possible to determine the concentration of CPR in the air at the workplace within the range from 0.002 to 0.04 mg/m (for an air sample of 120 L). The method is based on the adsorption of pararosaniline hydrochloride present in the workplace air on a polypropylene filter, eluting the substance deposited on the filter with methanol and analysing the solution thus obtained using high-performance liquid chromatography with a diode array detector (wavelength of 544 nm). Using an Ultra C18 (250 mm length) chromatographic column at a temperature of 23 °C and the mobile phase of methanol:0.1% phosphoric acid(V) (95:5, v/v) at flow rate of 0.6 mL/min makes it possible to determine the content of pararosaniline hydrochloride in the presence of aniline, nitrobenzene and 4-tolylamine. Limit of detection and limit of quantification were 0.17 ng/mL and 0.51 ng/mL, respectively.
盐酸副玫瑰苯胺（CPR）是一种用于给纸张、皮革和天然及人造纤维染色的染料。它也被用于分析和微生物学实验室。它是一种 1B 类致癌物质。在分析化学中，它被用于检测以下物质：溴酸盐、甲醛、臭氧、亚硫酸盐和二氧化硫。CPR 是一种在微生物学中常用的染料，用于染色制剂、染色细菌、抗体或其他生物。在波兰，约有 800 名员工接触过这种物质。由于缺乏测定工作场所空气中盐酸副玫瑰苯胺的方法，无法评估工人接触该物质的职业暴露情况。出于这个原因，已经开发出一种测定方法，可以在空气中测定盐酸副玫瑰苯胺。该方法可在工作场所空气中确定 CPR 的浓度，范围为 0.002 至 0.04mg/m³（120L 空气样本）。该方法基于吸附工作场所空气中存在的盐酸副玫瑰苯胺，用甲醇洗脱沉积在滤器上的物质，然后用高效液相色谱法与二极管阵列检测器（波长 544nm）分析所得溶液。在 23°C 的温度下使用 Ultra C18（250mm 长）色谱柱，流动相为甲醇：0.1%磷酸（V）（95:5，v/v），流速为 0.6mL/min，可以在存在苯胺、硝基苯和 4-甲苯胺的情况下测定盐酸副玫瑰苯胺的含量。检测限和定量限分别为 0.17ng/mL 和 0.51ng/mL。
