Pahumunto Nuntiya, Sophatha Benchamat, Piwat Supatcharin, Teanpaisan Rawee
Common Oral Diseases and Epidemiology Research Center and the Department of Stomatology, Faculty of Dentistry, Prince of Songkla University, Hat-Yai, 90112, Thailand.
Common Oral Diseases and Epidemiology Research Center and the Department of Preventive Dentistry, Faculty of Dentistry, Prince of Songkla University, Hat-Yai, 90112, Thailand.
J Dent Sci. 2019 Jun;14(2):178-184. doi: 10.1016/j.jds.2019.01.008. Epub 2019 Mar 27.
BACKGROUND/PURPOSE: Our previous study revealed that probiotic SD1 could reduce mutans streptococci as evaluated by cultivation-method as well as stimulate innate immunity. This study aimed to further investigate the effect of the probiotic on various oral bacteria by real-time PCR and salivary IgA levels.
Forty children were included by randomization from either probiotic or control group in the previous study. The probiotic or control received milk-powder with or without . SD1, respectively once daily for 6 months. Saliva were collected at baseline 3-, 6-, and 12-months and were evaluated for total bacteria, total lactobacilli (TL), (LP/LC), total streptococci (TS) and using the real-time PCR. The salivary IgA (sIgA) was examined using the ELISA method.
All target bacterial levels were not significantly different at baseline in both groups. After milk-powder consumption, TL and LP/LC levels were significantly increased in the probiotic group, whereas TS and levels were significantly decreased compared to baseline. TS and levels were significantly lower, while the sIgA was greater in the probiotic compared to the control group. In the probiotic group, a positive correlation was found between LP/LC and sIgA, while negative correlations were observed between TS or levels and sIgA.
SD1 could control level and could stimulate sIgA. Results indicate that the SD1 strain may have a benefit for prevention of dental caries.
背景/目的：我们之前的研究表明，益生菌SD1通过培养法评估可减少变形链球菌，并能刺激先天免疫。本研究旨在通过实时定量聚合酶链反应（real-time PCR）和唾液免疫球蛋白A（IgA）水平进一步研究该益生菌对各种口腔细菌的影响。
从之前研究的益生菌组或对照组中随机纳入40名儿童。益生菌组或对照组分别每日一次接受含或不含SD1的奶粉，持续6个月。在基线、3个月、6个月和12个月时收集唾液，使用实时定量聚合酶链反应评估总细菌、总乳酸菌（TL）、嗜酸乳杆菌/卷曲乳杆菌（LP/LC）、总链球菌（TS）和变异链球菌水平。使用酶联免疫吸附测定法检测唾液IgA（sIgA）。
两组在基线时所有目标细菌水平均无显著差异。食用奶粉后，益生菌组的TL和LP/LC水平显著升高，而TS和变异链球菌水平与基线相比显著降低。与对照组相比，益生菌组的TS和变异链球菌水平显著更低，而sIgA更高。在益生菌组中，LP/LC与sIgA之间呈正相关，而TS或变异链球菌水平与sIgA之间呈负相关。
SD1可控制变异链球菌水平并能刺激sIgA。结果表明，SD1菌株可能对预防龋齿有益。
