School of Food and Health, Beijing Technology and Business University, Beijing 100024, China.
Key Laboratory of Precision Nutrition and Food Quality, Department of Nutrition and Health, China Agricultural University, Beijing 100083, China.
Nutrients. 2023 Jul 26;15(15):3316. doi: 10.3390/nu15153316.
Dental caries is a common and multifactorial biofilm disease that is associated with dietary habits and microbiota. Among the various pathogens inducing caries, is the most extensively studied. Promoting oral health with probiotics has gained considerable attention. () strains were reported to modulate the gut microbiota and enhance host resistance to disease. Our previous research has found that ET-22 (ET-22) could inhibit biofilms in vitro. However, the preventive effect in vivo and functional mechanism of ET-22 on dental caries were unclear. In this study, the preventive effects of ET-22 on dental caries in mice were checked. Meanwhile, the functional mechanism of ET-22 was further investigated. Results showed that the supplementation of ET-22 in drinking water significantly improved the caries scoring of mice. The microbiota of dental plaques revealed that the live and heat-killed ET-22 similarly regulated the microbial structure in plaque biofilms. Functional prediction of PICRUSt showed that the addition of live and heat-killed ET-22 may inhibit biofilm formation. By the in vitro trials, the live and heat-killed ET-22 indeed inhibited the construction of biofilms and EPS productions of biofilms. This evidence suggests that ET-22 can restrain dental caries by regulating the microbiota of dental plaques and inhibiting biofilm formation, which may be partly mediated by the body components of ET-22.
龋齿是一种常见的多因素生物膜疾病，与饮食习惯和微生物群有关。在引起龋齿的各种病原体中， 是研究最多的。用益生菌促进口腔健康引起了相当大的关注。 () 菌株被报道可调节肠道微生物群并增强宿主对疾病的抵抗力。我们之前的研究发现 ET-22（ET-22）可以抑制 体外生物膜。然而，ET-22 对龋齿的体内预防作用及其功能机制尚不清楚。在这项研究中，检查了 ET-22 对小鼠龋齿的预防作用。同时，进一步研究了 ET-22 的功能机制。结果表明，饮用水中补充 ET-22 可显著改善小鼠的龋齿评分。牙菌斑的微生物群表明，活菌和热灭活 ET-22 同样调节了菌斑生物膜中的微生物结构。 PICRUSt 的功能预测表明，添加活菌和热灭活 ET-22 可能抑制生物膜形成。通过体外试验，活菌和热灭活 ET-22 确实抑制了 生物膜的构建和生物膜中 EPS 的产生。这一证据表明，ET-22 通过调节牙菌斑微生物群和抑制生物膜形成来抑制龋齿，这可能部分是由 ET-22 的机体成分介导的。
