Dipartimento di Farmacia-Scienze del Farmaco, Università degli Studi di Bari Aldo Moro, via Orabona 4, 70125 Bari, Italy.
Molecules. 2019 Jun 17;24(12):2251. doi: 10.3390/molecules24122251.
Preparations from the dried tubers of (Burch.) DC ex Meisn, commonly known as devil's claw, are mainly used in modern medicine to relieve joint pain and inflammation in patients suffering from rheumatic and arthritic disorders. This paper describes for the first time the chemical profile of a commercial spagyric tincture (named 019) prepared from the roots of the plant. For comparison purposes, a commercial not-spagyric devil's claw tincture (NST) was also analyzed. Chemical investigation of the content of specialized metabolites in the three samples indicated that harpagoside was the main compound, followed by the two isomers acteoside and isoacteoside. Compositional consistence over time was obtained by the chemical fingerprinting of another spagyric tincture (named 014) from the same producer that was already expired according to the recommendation on the label of the product. The two spagyric preparations did not show significant compositional differences as revealed by HPLC and MS analyses, except for a decrease in harpagide content in the expired 014 tincture. Moreover, their antioxidant capacities as assessed by 2,2'-di-phenyl-1-picrylhydrazyl (DPPH) and 2.2'-azin-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) methods resulted in very similar IC50 values. The expired 014 tincture showed instead a lower IC50 value compared to the 019 and NST tinctures with the ferric reducing antioxidant potential (FRAP) assay, indicating a higher ferric-reducing antioxidant ability. Overall, these results indicated that the two preparations could generally maintain good stability and biological activity at least for the four years from the production to the expiration date.
从（Burch.）DC ex Meisn 的干燥块茎中制备的制剂，通常被称为魔鬼爪，主要用于现代医学，以缓解患有风湿和关节炎疾病的患者的关节疼痛和炎症。本文首次描述了一种商业 spagyric 酊剂（命名为 019）的化学特征，该酊剂是从植物的根部制备的。为了比较目的，还分析了一种商业非 spagyric 魔鬼爪酊剂（NST）。对三种样品中专门代谢物含量的化学研究表明，哈帕苷是主要化合物，其次是两种异构体哈帕苷和异哈帕苷。通过同一生产商的另一种 spagyric 酊剂（命名为 014）的化学指纹分析获得了时间上的组成一致性，该酊剂根据产品标签上的建议已经过期。高效液相色谱和 MS 分析表明，两种 spagyric 制剂没有显示出明显的组成差异，除了过期的 014 酊剂中哈帕苷含量降低。此外，它们的抗氧化能力，如 2,2'-二苯基-1-苦基肼基（DPPH）和 2.2'-偶氮-双（3-乙基苯并噻唑啉-6-磺酸）（ABTS）方法评估，导致非常相似的 IC50 值。相反，过期的 014 酊剂的 IC50 值低于 019 酊剂和 NST 酊剂，这表明其具有更高的铁还原抗氧化能力。总的来说，这些结果表明，这两种制剂至少在从生产到过期的四年内通常可以保持良好的稳定性和生物活性。
