卵泡刺激素 (FSHβ) 基因多态性与獭兔繁殖性状的关联。

Follicle-stimulating hormone (FSHβ) gene polymorphisms and associations with reproductive traits in Rex rabbits.

机构信息

College of Animal Science and Veterinary Medicine, Shanxi Agricultural University, Taigu 030801, China; Institute of Animal Husbandry and Veterinary Medicine, Shanxi Academy of Agricultural Sciences, Taiyuan 030006, China.

UWA Institute of Agriculture, University of Western Australia, 35 Stirling Highway, Crawley, WA 6009, Australia.

出版信息

Anim Reprod Sci. 2019 Aug;207:36-43. doi: 10.1016/j.anireprosci.2019.05.013. Epub 2019 May 29.

DOI:10.1016/j.anireprosci.2019.05.013

PMID:31213331

Abstract

Follicle-stimulating hormone (FSH) stimulates granulosa cell proliferation and controls the development and maturation of oocytes. In this study with Rex rabbit, there was exploration of the relationships between reproductive traits and SNPs and haplotypes of FSHβ, and tested whether FSHβ SNPs would segregate between two lines, using 70 females from the White line and 100 females from the Beaver line. Three SNPs (FSH2, FSH30, FSH31) in exon1 and exon3 of FSHβ were strongly associated with reproductive traits in the combined population. For FSH2, the GG variant was associated with greater (P <  0.05) values for total number born (TNB) and number born alive (NBA), compared to those with the TT variant. For FSH30, for the AA variant there was greater (P <  0.05) values for TNB and NBA than with the GG and AG variants. For FSH31, the GG variant was associated with greater (P <  0.05) values for TNB, litter weight at birth (LWB) and litter size at 21 days of age (LS21), than the AG variant, and with greater (P < 0.01) values for NBA than the AG variant. When analyzed separately, FSH2 SNP was associated with LW21 in the Beaver line (P <  0.05), whereas FSH30 and FSH31 SNPs were associated with TNB and NBA in the White line (P <  0.05). It is concluded that genetic variation in FSHβ gene is associated with reproductive traits in the Rex rabbit, therefore, the FSH2, FSH30 and FSH31 SNPs can be used as molecular markers in genetic selection of rabbits.

摘要

卵泡刺激素（FSH）刺激颗粒细胞增殖，并控制卵母细胞的发育和成熟。在这项雷克斯兔的研究中，探讨了生殖性状与 FSHβ基因的 SNP 和单倍型之间的关系，并检测了 FSHβ SNP 是否会在两个品系之间分离，使用了来自 White 品系的 70 只雌性和 Beaver 品系的 100 只雌性。FSHβ 外显子 1 和外显子 3 中的三个 SNP（FSH2、FSH30、FSH31）与合并群体的生殖性状密切相关。对于 FSH2，与 TT 变异相比，GG 变异与总出生数（TNB）和活产数（NBA）的更大（P<0.05）值相关。对于 FSH30，AA 变异的 TNB 和 NBA 值大于 GG 和 AG 变异。对于 FSH31，与 AG 变异相比，GG 变异与 TNB、出生时窝重（LWB）和 21 日龄窝重（LS21）的更大（P<0.05）值相关，与 AG 变异相比，NBA 的更大（P<0.01）值相关。单独分析时，FSH2 SNP 与 Beaver 系的 LW21 相关（P<0.05），而 FSH30 和 FSH31 SNP 与 White 系的 TNB 和 NBA 相关（P<0.05）。结论是，FSHβ基因的遗传变异与雷克斯兔的生殖性状相关，因此，FSH2、FSH30 和 FSH31 SNP 可作为兔遗传选择的分子标记。