用于组装染料颗粒等离子体的胶体银纳米三角形的密度梯度选择

Density Gradient Selection of Colloidal Silver Nanotriangles for Assembling Dye-Particle Plasmophores.

作者信息

Oliveira-Silva Rui, Sousa-Jerónimo Mariana, Botequim David, Silva Nuno J O, Prazeres Duarte M F, Paulo Pedro M R

机构信息

iBB-Institute for Bioengineering and Biosciences, Instituto Superior Técnico, Universidade de Lisboa, Av. Rovisco Pais 1, 1049-001 Lisboa, Portugal.

Departamento de Física and CICECO, Aveiro Institute of Materials, Universidade de Aveiro, 3810-193 Aveiro, Portugal.

出版信息

Nanomaterials (Basel). 2019 Jun 18;9(6):893. doi: 10.3390/nano9060893.

DOI:10.3390/nano9060893

PMID:31216629

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6631754/

Abstract

A simple method based on sucrose density gradient centrifugation is proposed here for the fractionation of colloidal silver nanotriangles. This method afforded particle fractions with surface plasmon resonances, spanning from red to infrared spectral ranges that could be used to tune optical properties for plasmonic applications. This feature was exemplified by selecting silver nanotriangle samples with spectral overlap with Atto-655 dye's absorption and emission in order to assemble dye-particle plasmophores. The emission brightness of an individual plasmophore, as characterized by fluorescence correlation spectroscopy, is at least 1000-fold more intense than that of a single Atto-655 dye label, which renders them as promising platforms for the development of fluorescence-based nanosensors.

摘要

本文提出了一种基于蔗糖密度梯度离心的简单方法，用于胶体银纳米三角形的分级分离。该方法得到了具有表面等离子体共振的颗粒级分，其范围从红色到红外光谱范围，可用于调节等离子体应用的光学性质。通过选择与Atto-655染料的吸收和发射光谱重叠的银纳米三角形样品来组装染料-颗粒等离子体激元，证明了这一特性。通过荧光相关光谱表征，单个等离子体激元的发射亮度比单个Atto-655染料标记至少强1000倍，这使其成为基于荧光的纳米传感器开发的有前景的平台。