Spiegelberg H L, Dainer P M
Clin Exp Immunol. 1979 Feb;35(2):286-95.
Fc receptors for IgG, IgM, IgE and the cell surface immunoglobulins (SIg) were analysed on lymphocytes from seventeen patients with chronic lymphatic leukaemia (CLL), one with lympho-sarcoma cell leukaemia (LSL), two each with hairy cell leukaemia (HCL), acute lymphatic leukaemia (ALL) and Sézary syndrome. Fc receptors for IgG and IgM were detected by rosette formation with ox erythrocytes (E) sensitized with rabbit IgG (EA) and IgM (EA) anti-E antibodies, respectively. Fc receptors for IgE were analysed either with E coated with glutaraldehyde coupled complexes consisting of rabbit Fab' fragments of anti-E antibodies and Fc fragments of an IgE myeloma protein (EA), or with aldehyde fixed E to which IgE was adsorbed. SIg of classes IgM, IgD, IgG and κ and λ light chain type were detected with E coated with complexes consisting of Fab'-anti-E and purified F(ab') fragments of specific goat antibodies. Lymphocytes of all patients with CLL, LSL and HCL had Fc receptors for IgG (65±15% EA, normal 22·0±5·8%). Ten patients had significant numbers of cells with IgM Fc receptors (37±22% EA, normal 1·2±1·5%) which were detected without overnight culturing of the lymphocytes. Lymphocytes of four patients (two CLL, one LSL, one HCL) had Fc receptors for IgE (22–88% EA, normal 1·8±0·7%). The cells of three of these four patients were also EA. The high numbers of rosetting cells indicated that individual lymphocytes must have carried more than one class of Fc receptors. The lymphocytes of the ALL and Sézary syndrome patients had few Fc receptor positive cells. Of the seventeen patients with CLL, twelve were SIgM and/or SIgD, only four κ or λ and one had no SIg. The cells of the LSL and one of the HCL patients were SIgM and SIgD, whilst the cells of the other HCL patient were SIgG, λ. None of the other patients had more than 10% SIgG cells. The ALL and Sézary syndrome patients had low numbers of SIg and Fc receptor positive cells. These data indicate that lymphocytes of patients with B-cell leukaemias can carry different classes of Fc receptors simultaneously; the different classes are found in a decreasing frequency of IgG>IgM>IgE.
对17例慢性淋巴细胞白血病（CLL）患者、1例淋巴肉瘤细胞白血病（LSL）患者、2例毛细胞白血病（HCL）患者、2例急性淋巴细胞白血病（ALL）患者和2例Sezary综合征患者的淋巴细胞进行了IgG、IgM、IgE的Fc受体以及细胞表面免疫球蛋白（SIg）分析。通过分别与用兔IgG（EA）和IgM（EA）抗E抗体致敏的牛红细胞（E）形成花环来检测IgG和IgM的Fc受体。用戊二醛偶联的复合物包被E来分析IgE的Fc受体，该复合物由抗E抗体的兔Fab′片段和IgE骨髓瘤蛋白的Fc片段组成（EA），或者用吸附了IgE的醛固定E来分析。用由Fab′-抗E和特异性山羊抗体的纯化F(ab′)片段组成的复合物包被E来检测IgM、IgD、IgG类以及κ和λ轻链型的SIg。所有CLL、LSL和HCL患者的淋巴细胞均有IgG的Fc受体（65±15% EA，正常为22.0±5.8%）。10例患者有大量带有IgM Fc受体的细胞（37±22% EA，正常为1.2±1.5%），这些细胞在未对淋巴细胞进行过夜培养的情况下即可检测到。4例患者（2例CLL、1例LSL、1例HCL）的淋巴细胞有IgE的Fc受体（22 - 88% EA，正常为1.8±0.7%）。这4例患者中的3例其细胞也是EA。大量的花环形成细胞表明单个淋巴细胞必定携带了不止一类Fc受体。ALL和Sezary综合征患者的淋巴细胞中Fc受体阳性细胞很少。17例CLL患者中，12例为SIgM和/或SIgD，仅4例为κ或λ，1例无SIg。LSL患者和1例HCL患者的细胞为SIgM和SIgD，而另1例HCL患者的细胞为SIgG、λ。其他患者中无一例SIgG细胞超过10%。ALL和Sezary综合征患者的SIg和Fc受体阳性细胞数量较少。这些数据表明B细胞白血病患者的淋巴细胞可同时携带不同类别的Fc受体；不同类别的Fc受体出现频率依次降低，即IgG>IgM>IgE。
