Division of Pharmacology & Toxicology, ICAR-Indian Veterinary Research Institute, Izatnagar, 243122, U.P, India.
Department of Entomology, SASRD, Medziphema Campus, 797106, NU, Nagaland, India.
Eur J Pharmacol. 2019 Sep 5;858:172466. doi: 10.1016/j.ejphar.2019.172466. Epub 2019 Jun 17.
Icariin is a flavonoid from plant belonging to the genus Epimedium, commonly known as Horny goat weed or Yin Yang Huo. The compound possesses multiple biological activities which are associated with the modulation of many signalling pathways, like NF-κB, Erk-p38-JNK, and release of various cytokines and growth factors. The present study determined wound healing potential of icariin in male Wistar rats. Icariin ointment (0%, 0.004%, 0.02%, 0.1% and 0.5%), was applied daily (b.i.d.) for 14 days on ≈ 400 mm cutaneous wound in different groups of rats. On day 14 post-wounding, 0.1% and 0.5% icariin treatment significantly (P < 0.01 and P < 0.001, respectively) increased wound contraction, as compared to control. Western blots revealed upregulation of IL-10 and downregulation of NF-κB and TNF-α. Increased expression of CD-31 showed abundance of microvessels in healing tissues after treatment with icariin. The MMP-2 and MMP-9 activities were reduced in icariin treated groups. Masson's trichrome staining revealed relatively better completion of re-epithelisation as well as increased deposition of well organised collagen fibres in the healing tissues compared to control. It is concluded that icariin has potential to accelerate cutaneous wound healing in rats.
朝藿定是一种黄酮类化合物，来源于淫羊藿属植物，俗称“淫羊藿”或“阴阳藿”。该化合物具有多种生物学活性，与多种信号通路的调节有关，如 NF-κB、Erk-p38-JNK 以及各种细胞因子和生长因子的释放。本研究旨在确定朝藿定对雄性 Wistar 大鼠的创伤愈合作用。朝藿定软膏（0%、0.004%、0.02%、0.1%和 0.5%）分别在不同组别的大鼠的约 400mm 皮肤伤口上每天（两次）使用 14 天。在创伤后第 14 天，与对照组相比，0.1%和 0.5%朝藿定治疗组的伤口收缩显著增加（P<0.01 和 P<0.001）。Western blot 显示，IL-10 表达上调，NF-κB 和 TNF-α表达下调。经朝藿定治疗后，CD-31 的表达增加，表明愈合组织中微血管丰富。朝藿定治疗组的 MMP-2 和 MMP-9 活性降低。Masson 三色染色显示，与对照组相比，朝藿定治疗组的再上皮化完成情况较好，且愈合组织中胶原纤维的沉积更加有序。综上所述，朝藿定具有促进大鼠皮肤创伤愈合的潜力。
