Department of Veterinary Pharmacology & Toxicology, COVSc. & A.H., CAU, Jalukie, 797110, Nagaland, India.
Division of Pharmacology & Toxicology, ICAR-Indian Veterinary Research Institute, Izatnagar, 243122, U.P, India.
J Tissue Viability. 2022 Feb;31(1):197-206. doi: 10.1016/j.jtv.2021.09.004. Epub 2021 Sep 21.
In diabetes, wound healing gets delayed due to various factors. Icariin, a flavonoid obtained from the plants of the Epimedium genus, exhibited anti-inflammatory, angiogenic, and matrix metalloproteinase-inhibiting effects to heal skin wounds in non-diabetic rats on topical application. Hence, we designed the present study to explore icariin's potential to heal cutaneous diabetic wounds in rats. Diabetes was induced by streptozotocin in male Wistar rats and they were divided into two groups after creating a skin wound of approximately 4 cm area. Simple ointment base and 0.04% icariin ointment were applied twice daily for 19 days in the control and the treatment group, respectively. The healing of the wound was assessed based on wound closure, the expression patterns of NF-κB, TNF-α, IL-10, CD31, MMP-2 and -9 activities, and collagen deposition on predetermined days after wound creation. Wounds treated with icariin showed a marked increase in per cent wound closure on different post-wounding days than diabetic control. Upregulation of IL-10 and decreased expressions of NF-κB and TNF-α were revealed in western blots, indicating an anti-inflammatory effect of icariin. Western blot, as well as immunohistochemistry, showed increased expression of CD31 on all days confirming the angiogenic effect of icariin in healing. Icariin treatment reduced MMP-2 and -9 activities and increased deposition of well-organized collagen. Results demonstrate that icariin reduced inflammation and improved angiogenesis and thus, it appears to possess the potential to enhance the healing of diabetic wounds.
在糖尿病中，由于各种因素，伤口愈合会延迟。淫羊藿素是从淫羊藿属植物中提取的一种黄酮类化合物，具有抗炎、血管生成和基质金属蛋白酶抑制作用，可在局部应用于非糖尿病大鼠时促进皮肤伤口愈合。因此，我们设计了本研究来探索淫羊藿素促进糖尿病大鼠皮肤伤口愈合的潜力。雄性 Wistar 大鼠通过链脲佐菌素诱导糖尿病，并在创建约 4 cm 面积的皮肤伤口后将其分为两组。对照组和治疗组大鼠分别每天两次涂抹普通软膏基质和 0.04%淫羊藿素软膏，持续 19 天。根据伤口闭合、NF-κB、TNF-α、IL-10、CD31、MMP-2 和 -9 活性以及胶原沉积的表达模式评估伤口愈合情况。与糖尿病对照组相比，用淫羊藿素治疗的伤口在不同的创伤后天数显示出明显增加的百分比伤口闭合。Western blot 显示 IL-10 上调，NF-κB 和 TNF-α 表达下调，表明淫羊藿素有抗炎作用。Western blot 和免疫组化显示 CD31 在所有天数的表达增加，证实了淫羊藿素在愈合过程中的血管生成作用。淫羊藿素治疗降低了 MMP-2 和 -9 的活性，并增加了有序胶原的沉积。结果表明，淫羊藿素减少了炎症，改善了血管生成，因此似乎具有增强糖尿病伤口愈合的潜力。
