Jayaswall Kuldip, Mahajan Pallavi, Singh Gagandeep, Parmar Rajni, Seth Romit, Raina Aparnashree, Swarnkar Mohit Kumar, Singh Anil Kumar, Shankar Ravi, Sharma Ram Kumar
Biotechnology Department, CSIR-Institute of Himalayan Bioresource Technology, Palampur, Himachal Pradesh, India, 176061.
Sci Rep. 2016 Jul 28;6:30412. doi: 10.1038/srep30412.
To unravel the molecular mechanism of defense against blister blight (BB) disease caused by an obligate biotrophic fungus, Exobasidium vexans, transcriptome of BB interaction with resistance and susceptible tea genotypes was analysed through RNA-seq using Illumina GAIIx at four different stages during ~20-day disease cycle. Approximately 69 million high quality reads were assembled de novo, yielding 37,790 unique transcripts with more than 55% being functionally annotated. Differentially expressed, 149 defense related transcripts/genes, namely defense related enzymes, resistance genes, multidrug resistant transporters, transcription factors, retrotransposons, metacaspases and chaperons were observed in RG, suggesting their role in defending against BB. Being present in the major hub, putative master regulators among these candidates were identified from predetermined protein-protein interaction network of Arabidopsis thaliana. Further, confirmation of abundant expression of well-known RPM1, RPS2 and RPP13 in quantitative Real Time PCR indicates salicylic acid and jasmonic acid, possibly induce synthesis of antimicrobial compounds, required to overcome the virulence of E. vexans. Compendiously, the current study provides a comprehensive gene expression and insights into the molecular mechanism of tea defense against BB to serve as a resource for unravelling the possible regulatory mechanism of immunity against various biotic stresses in tea and other crops.
为了揭示茶树抵御由专性活体营养型真菌外担菌（Exobasidium vexans）引起的赤星病（BB）的分子机制，在约20天的病程中，于四个不同阶段通过RNA测序（RNA-seq），利用Illumina GAIIx对赤星病与抗性和感病茶树基因型相互作用的转录组进行了分析。大约6900万个高质量 reads 被从头组装，产生了37790个独特转录本，其中超过55%具有功能注释。在抗性基因型（RG）中观察到149个差异表达的防御相关转录本/基因，即防御相关酶、抗性基因、多药耐药转运蛋白、转录因子、反转录转座子、metacaspases和伴侣蛋白，表明它们在抵御赤星病中发挥作用。从拟南芥预先确定的蛋白质-蛋白质相互作用网络中，在这些候选物中鉴定出位于主要枢纽的假定主调控因子。此外，定量实时PCR证实了著名的RPM1、RPS2和RPP13的大量表达，表明水杨酸和茉莉酸可能诱导合成克服外担菌毒力所需的抗菌化合物。简而言之，本研究提供了全面的基因表达情况，并深入了解了茶树抵御赤星病的分子机制，为揭示茶树和其他作物对各种生物胁迫的免疫可能调控机制提供了资源。
