College of Light Industry and Food Engineering, Guangxi University, 100 Daxue Road, Nanning 530004, People's Republic of China; College of Food and Biological Technology, Guangxi Vocational and Technical College, Ming Yang Industrial Park Jiangnan District, Nanning 530226, People's Republic of China.
College of Light Industry and Food Engineering, Guangxi University, 100 Daxue Road, Nanning 530004, People's Republic of China.
Genomics. 2020 Jan;112(1):769-773. doi: 10.1016/j.ygeno.2019.05.015. Epub 2019 Jun 18.
B. longum LTBL16 is a potential probiotic strain that was isolated from healthy centenarians in Bama, China. In vitro experiments show that B. longum LTBL16 has a strong antioxidant activity and the complete genome of B. longum LTBL16 was sequenced in this work. The genome consists of one 2,430,682 bp circular chromosome that is plasmid free. The circular chromosome has a GC content of 61.23% and contains 2071 coding sequences (CDSs), 4 rRNA manipulators and 55 tRNA coding genes. Genetic analysis showed that at least five protein-coding genes were associated with antioxidant activity, and the abundance of these genes may be related to free radical scavenging rates and oxygen tolerance. In addition, the safety of B. longum LTBL16 was evaluated using a virulence factor database and antibiotic resistance gene database. The results indicate that B. longum LTBL16 has the good potential for the development and utilization as a probiotic.
长双歧杆菌 LTBL16 是从中国巴马的健康百岁老人中分离出来的一种有潜力的益生菌菌株。体外实验表明，长双歧杆菌 LTBL16 具有很强的抗氧化活性，本工作对其全基因组进行了测序。基因组由一个 2,430,682 bp 的环形染色体组成，不含质粒。环形染色体的 GC 含量为 61.23%，包含 2071 个编码序列（CDS）、4 个 rRNA 操纵子和 55 个 tRNA 编码基因。遗传分析表明，至少有五个与抗氧化活性相关的蛋白编码基因，这些基因的丰度可能与自由基清除率和耐氧性有关。此外，还使用了毒力因子数据库和抗生素耐药基因数据库来评估长双歧杆菌 LTBL16 的安全性。结果表明，长双歧杆菌 LTBL16 具有作为益生菌开发和利用的良好潜力。
