通过基因组学研究婴儿源BF_15的胃肠道适应性和抗氧化功能机制。
Mechanism of gastrointestinal adaptability and antioxidant function of infant-derived BF_15 through genomics.
作者信息
Wang Xinyu, Zhang Na, Li Dongyao, Wang Miaoshu, Li Chen, Tian Hongtao
机构信息
College of Food Science and Technology, Hebei Agricultural University, Baoding, Hebei 071000 China.
College of Biochemistry and Environmental Engineering, Baoding University, Baoding, Hebei 071000 China.
出版信息
Food Sci Biotechnol. 2022 Aug 5;31(11):1451-1462. doi: 10.1007/s10068-022-01132-w. eCollection 2022 Oct.
UNLABELLED
is an essential probiotic in the human gastrointestinal tract. BF_15, a functional probiotic isolated from the feces of breast-fed infants, has been reported in many in vitro and in vivo studies with strong gastrointestinal adaptability and outstanding anti-oxidative activities. Therefore, the whole genome of BF_15 was sequenced. Several genes, encoding the gastrointestinal adaptability-related proteins, were identified, including genes related to gastrointestinal environment-induced stress resistance, adhesive performance, and ability to transport and metabolize resistant starch and oligosaccharides. Genes related to alleviating oxidative stress were also found. Further functional verification was carried out by RT-qPCR on the 10 and 12 key adhesion and antioxidant genes. Overall, this study might provide a critical basis for BF_15 as a potential candidate for probiotics.
SUPPLEMENTARY INFORMATION
The online version contains supplementary material available at 10.1007/s10068-022-01132-w.
未标记
是人类胃肠道中的一种必需益生菌。BF_15是一种从母乳喂养婴儿粪便中分离出的功能性益生菌，许多体外和体内研究均报道其具有很强的胃肠道适应性和出色的抗氧化活性。因此，对BF_15的全基因组进行了测序。鉴定出了几个编码胃肠道适应性相关蛋白的基因，包括与胃肠道环境诱导的应激抗性、黏附性能以及转运和代谢抗性淀粉和低聚糖能力相关的基因。还发现了与减轻氧化应激相关的基因。通过RT-qPCR对10个和12个关键黏附及抗氧化基因进行了进一步的功能验证。总体而言，本研究可能为BF_15作为益生菌的潜在候选物提供关键依据。
补充信息
在线版本包含可在10.1007/s10068-022-01132-w获取的补充材料。
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