Department of Obstetrics and Gynecology, School of Medicine, Muğla Sıtkı Koçman University, Mugla 48000, Turkey.
Department of Plastic, Reconstructive and Aesthetic Surgery, School of Medicine, Muğla Sıtkı Koçman University, Mugla 48000, Turkey.
Gene. 2019 Aug 30;711:143937. doi: 10.1016/j.gene.2019.06.027. Epub 2019 Jun 19.
BACKGROUND & OBJECTIVES: Vaginal atrophy is characterized by thinning of vaginal epithelial layers and decreased local blood flow. We aimed to evaluate the regenerative effects of Adipose derived mesenchymal stem cells (ADMSC) and Bone marrow derived mesenchymal stem cells (BMDSC) on vaginal atrophy in rat menopause model.
Rats were randomly divided into 4 (four) groups: sham, control, ADMSC, BMDSC. Vaginal epithelial thickness, structure of the lamina propria, blood vessels in the lamina propria, collagen deposition, and muscle structure were evaluated. Anti ER α, VEGF, VEGFR 1, Bax and bcl-2 antibodies were analyzed. Beta actin gene was used as endogenous control. Genetical differences among the groups were compared by using Kruskal Wallis and Mann Whitney U test. p < 0.05 was regarded as statistically significant.
Epithelial thickness of ADMSC group was higher than control group, but less than sham group Epithelial thickness of BMDSC group was less than sham group. Lamina propria and muscle tissue of ADMSC and BMDSC groups were found to be similar to sham group. VEGFR-1, VEGF, Bax and ER-α staining levels were higher in ADMSC and BMDSC groups than control group. ADMSC group stained stronger with VEGFR-1 and VEGF than BMDSC group. Bcl-2 staining level was increased in ADMSC applied group. No statistically significant difference was detected in Bax and Bcl-2 genes and Bax-/Bcl-2 ratio.
Although genetic expression might have ended and could not be significantly demonstrated, histological and immunohistochemical results favor ADMSC application in vaginal atrophy rather than BMDSC.
阴道萎缩的特征是阴道上皮层变薄和局部血流量减少。我们旨在评估脂肪间充质干细胞（ADMSC）和骨髓间充质干细胞（BMDSC）对大鼠绝经后模型阴道萎缩的再生作用。
将大鼠随机分为 4 组：假手术组、对照组、ADMSC 组、BMDSC 组。评估阴道上皮厚度、固有层结构、固有层血管、胶原沉积和肌肉结构。分析抗 ERα、VEGF、VEGFR1、Bax 和 bcl-2 抗体。使用内参基因β-肌动蛋白。使用 Kruskal-Wallis 和 Mann-Whitney U 检验比较组间的遗传差异。p<0.05 被认为具有统计学意义。
ADMSC 组的上皮厚度高于对照组，但低于假手术组；BMDSC 组的上皮厚度低于假手术组。ADMSC 和 BMDSC 组的固有层和肌肉组织与假手术组相似。ADMSC 和 BMDSC 组的 VEGFR-1、VEGF、Bax 和 ER-α染色水平高于对照组。ADMSC 组的 VEGFR-1 和 VEGF 染色强度强于 BMDSC 组。ADMSC 应用组的 Bcl-2 染色水平增加。Bax 和 Bcl-2 基因及 Bax/Bcl-2 比值无统计学差异。
尽管基因表达可能已经结束，并且无法得到显著证明，但组织学和免疫组织化学结果支持 ADMSC 应用于阴道萎缩，而不是 BMDSC。
