Hendricks R L, Fishman G A
Department of Ophthalmology, Lions of Illinois Eye Research Institute, University of Illinois School of Medicine, Chicago 60612.
Exp Eye Res. 1987 Dec;45(6):923-31. doi: 10.1016/s0014-4835(87)80106-9.
Other investigators have reported deficient production of interferon-gamma (IFN-gamma) and reduced expression of class II major histocompatibility (HLA-DR) antigens on monocytes from patients with retinitis pigmentosa (RP). Our previous investigation did not demonstrate deficient IFN-gamma production by lymphocytes from patients with RP. We have extended our previous study by determining the frequency of HLA-DR-positive monocytes in the peripheral blood of well-defined groups of RP patients and by including a larger sample size of patients and subdividing the autosomal dominant and recessive subpopulations. Our present results confirm and extend our previous finding that lymphocytes from patients with RP are not deficient in the production of IFN-gamma as assessed with a commercially available radioimmunoassay test kit. In addition, using two-color immunofluorescence staining and flow cytometry, we also demonstrated normal expression of HLA-DR antigens on monocytes from these patients. In both this and our previous study, using techniques employed in our laboratory, we have been unable to detect significant cell-mediated immune abnormalities in a large and well-characterized group of RP patients.
其他研究人员报告称，视网膜色素变性（RP）患者的单核细胞中干扰素-γ（IFN-γ）产生不足，且II类主要组织相容性（HLA-DR）抗原的表达降低。我们之前的研究并未证明RP患者的淋巴细胞产生IFN-γ不足。我们通过确定明确分组的RP患者外周血中HLA-DR阳性单核细胞的频率，并纳入更大样本量的患者，并将常染色体显性和隐性亚群细分，扩展了我们之前的研究。我们目前的结果证实并扩展了我们之前的发现，即使用市售放射免疫分析测试试剂盒评估时，RP患者的淋巴细胞产生IFN-γ并不不足。此外，使用双色免疫荧光染色和流式细胞术，我们还证明了这些患者单核细胞上HLA-DR抗原的正常表达。在本研究和我们之前的研究中，使用我们实验室采用的技术，我们在一大群特征明确的RP患者中均未检测到明显的细胞介导免疫异常。
