Villa M L, Valenti F, Mantovani M
Cattedra di Immunologia, University of Milan, Italy.
Immunology. 1988 Jan;63(1):93-7.
The effect on NK activity of two oxygenase inhibitors, indomethacin which is cyclo-oxygenase-specific at 10(-6) M and BW755C which blocks both cyclo- and lipo-oxygenase, has been analysed. The NK activity of human peripheral blood mononuclear cells (PBMC) has been studied in a 18-hr 51Cr-release assay. PBMC were incubated with the drugs for different periods of time before and during NK assay in order to analyse the correlations between the time of incubation and the modulation of NK activity. The results show that the inhibition of the cyclo-oxygenase alone, by indomethacin, enhances the NK activity if the drug is added both during and prior to the assay. In the latter case the enhancement is time dependent, reaching a maximum level after 18 hr of incubation. The simultaneous inhibition of lipo- and cyclo-oxygenase by BW755C has a rather more complex effect. The NK activity is similar to that of untreated cells after 6 hr incubation with BW755C, but is inhibited or greatly enhanced after 4 and 18 hr of incubation, respectively. These effects seem largely independent of the presence of monocytes because they can be observed with non-adherent PBMC (NA-PBMC).
分析了两种加氧酶抑制剂对自然杀伤（NK）活性的影响，这两种抑制剂分别是10⁻⁶ M时具有环氧化酶特异性的吲哚美辛和同时阻断环氧化酶与脂氧化酶的BW755C。在一项18小时的⁵¹Cr释放试验中研究了人外周血单个核细胞（PBMC）的NK活性。为了分析孵育时间与NK活性调节之间的相关性，在NK试验之前和期间，将PBMC与药物孵育不同的时间段。结果表明，如果在试验期间和试验之前都加入吲哚美辛，单独抑制环氧化酶会增强NK活性。在后一种情况下，增强作用是时间依赖性的，孵育18小时后达到最大水平。BW755C同时抑制脂氧化酶和环氧化酶具有更为复杂的作用。与BW755C孵育6小时后，NK活性与未处理细胞的相似，但分别在孵育4小时和18小时后受到抑制或大幅增强。这些效应似乎很大程度上与单核细胞的存在无关，因为在非贴壁PBMC（NA-PBMC）中也能观察到。
