National Centre of Excellence in Analytical Chemistry, University of Sindh, Jamshoro, 76080, Pakistan; Beijing Advanced Innovation Centre for Soft Matter Science and Engineering, Beijing University of Chemical Technology, Beijing, 100029, China; Interface Analysis Centre, School of Physics, University of Bristol, Bristol, BS8 1TL, UK.
Institute of Chemistry, Shah Abdul Latif University Khairpur, 66020, Pakistan.
Biosens Bioelectron. 2019 Sep 15;141:111331. doi: 10.1016/j.bios.2019.111331. Epub 2019 Jun 8.
This study describes the construction of highly-sensitive photo-electrochemical (PEC) immunosensor for the detection of neuron-specific enolase (NSE). The biosensing platform is comprised of photo-active NiWO nanostructures, in-situ-grown over a conductive substrate (indium tin oxide) using a low-temperature template-based co-precipitation approach. The discussed approach enables the formation of discrete, yet morphologically-analogous, nanostructures with complete coverage (pinhole-free) of the electrode surface. The in-situ-grown nanostructure possess dense population with sharp saw-blade like morphological features that can support substantial immobilisation of anti-NSE agent. The constructed platform demonstrated excellent photo-catalytic activity towards uric acid (UA) which served as the base for the Electrochemical -mechanism (EC) based PEC inhibition sensing. The detection of NSE, relied on its obstruction in analytical signal observed for the photo-oxidation of UA after binding to the electrode surface via protein-antibody interaction. The constructed PEC immunosensor exhibits signal sensitivity up to 0.12 ng mL of NSE with excellent signal reproducibility and electrode replicability. Moreover, the constructed platform was successfully used for NSE determination in human serum samples.
本研究描述了一种用于检测神经元特异性烯醇化酶（NSE）的高灵敏度光电化学（PEC）免疫传感器的构建。该生物传感平台由光电活性的 NiWO 纳米结构组成，通过低温模板辅助共沉淀方法在导电基底（氧化铟锡）上原位生长。所讨论的方法能够形成离散的、形态相似的纳米结构，且电极表面具有完整的覆盖（无针孔）。原位生长的纳米结构具有密集的、具有锋利锯齿状形态特征的种群，可支持大量的抗 NSE 试剂固定化。构建的平台对尿酸（UA）表现出优异的光催化活性，UA 可用作基于电化学机制（EC）的 PEC 抑制传感的基础。NSE 的检测依赖于其在与电极表面通过蛋白质-抗体相互作用结合后对 UA 的光氧化分析信号的阻碍。构建的 PEC 免疫传感器对 NSE 的检测灵敏度高达 0.12ng/mL，具有出色的信号重现性和电极可重复性。此外，该构建平台成功地用于人血清样品中 NSE 的测定。
