Amani Jafar, Maleki Mehdi, Khoshroo Alireza, Sobhani-Nasab Ali, Rahimi-Nasrabadi Mehdi
Applied Microbiology Research Center, Systems Biology and Poisonings Institute, Baqiyatallah University of Medical Sciences, Tehran, Iran.
Young Researchers and Elite Club, Arak Branch, Islamic Azad University, Arak, Iran.
Anal Biochem. 2018 May 1;548:53-59. doi: 10.1016/j.ab.2018.02.024. Epub 2018 Feb 24.
In this work, a label-free electrochemical immunosensor was constructed on the base of poly p-phenylenediamine (PPD) and GR nanocomposite (PPD-GR). Screen-printed electrodes modified with PPD-GR nanocomposite and applied to advance enzyme-free and label free electrochemical immunosensor for detection of protein biomarker neuron-specific enolase (NSE). It was found that the PPD-GR nanocomposite exhibits excellent electrocatalytic activity towards ascorbic acid (AA) oxidation as analytical signal based on EC' mechanism. Due to the excellent electrocatalytic activity of PPD-GR nanocomposite, determination of NSE antigen was based on its obstruction to the electrocatalytic oxidation of AA after binding to the surface of electrode through interaction with the anti-NSE. The proposed immunosensor exhibited a wide linear range of 1.0-1000 ng mL, with a low detection limit of 0.3 ng mL. Furthermore, the proposed immunosensor were successfully used for the determination of NSE antigen in human serum samples.
在本工作中，基于聚对苯二胺（PPD）和氧化石墨烯（GR）纳米复合材料（PPD-GR）构建了一种无标记电化学免疫传感器。用PPD-GR纳米复合材料修饰丝网印刷电极，并将其应用于改进的无酶、无标记电化学免疫传感器，用于检测蛋白质生物标志物神经元特异性烯醇化酶（NSE）。研究发现，基于EC'机制，PPD-GR纳米复合材料对抗坏血酸（AA）氧化表现出优异的电催化活性，可作为分析信号。由于PPD-GR纳米复合材料具有优异的电催化活性，NSE抗原的测定基于其与抗NSE相互作用结合到电极表面后对AA电催化氧化的阻碍作用。所提出的免疫传感器线性范围宽，为1.0 - 1000 ng/mL，检测限低至0.3 ng/mL。此外，所提出的免疫传感器成功用于人血清样品中NSE抗原的测定。
