Institute of Human Virology, Sun Yat-Sen University, Guangzhou, China.
Department of Biochemistry and Molecular Biology, Zhongshan School of Medicine, Sun Yat-Sen University, Guangzhou, China.
J Immunol Res. 2019 May 22;2019:2431617. doi: 10.1155/2019/2431617. eCollection 2019.
Th17 activity has been implicated in systemic lupus erythematosus (SLE), which is a systemic autoimmune disease with a typical clinical manifestation of lupus nephritis (LN). Retinoic acid receptor-related orphan receptor gamma t (RORt) has been shown to be important for Th17 differentiation. In this study, we evaluated the inhibition of RORt activity by 3-acetyloxy-oleanolic acid (AOA), a small molecule isolated from the root of , a traditional herb belonging to South China. We demonstrated that AOA can inhibit RORt activity and prevent SLE pathogenesis in a pristane-induced LN model. The results showed that AOA decreased RORt transcription activity in a reporter assay and prevented Th17 differentiation . studies showed that AOA treatment decreased serum anti-dsDNA antibody and alleviated renal pathologic damage as well as antibody complex accumulation in the pristane-induced LN model. These results demonstrated that AOA can improve the clinical manifestation of LN, indicating potential application in SLE therapy.
Th17 活性与系统性红斑狼疮（SLE）有关，SLE 是一种系统性自身免疫性疾病，其典型临床表现为狼疮肾炎（LN）。维甲酸受体相关孤儿受体γ t（RORγ t）对于 Th17 分化非常重要。在这项研究中，我们评估了从 根部分离得到的小分子 3-乙酰氧基齐墩果酸（AOA）对 RORγ t 活性的抑制作用， 是华南地区的一种传统草药。我们证明 AOA 可以抑制 RORγ t 活性，并预防经正十六烷诱导的 LN 模型中的 SLE 发病机制。结果表明，AOA 在报告基因检测中降低了 RORγ t 的转录活性，并阻止了 Th17 分化。 研究表明，AOA 治疗降低了血清抗 dsDNA 抗体，并减轻了正十六烷诱导的 LN 模型中的肾脏病理损伤和抗体复合物积聚。这些结果表明 AOA 可以改善 LN 的临床表现，表明其在 SLE 治疗中有潜在的应用。
