Reynoso Glennys V, Shannon John P, Americo Jeffrey L, Gibbs James, Hickman Heather D
Viral Immunity and Pathogenesis Unit, Laboratory of Clinical Immunology and Microbiology (LCIM), National Institute of Allergy and Infectious Diseases (NIAID), National Institutes of Health (NIH), Bethesda, MD, USA.
Genetic Engineering Section, Laboratory of Viral Diseases (LVD), NIAID, NIH, Bethesda, MD, USA.
Methods Mol Biol. 2019;2023:287-299. doi: 10.1007/978-1-4939-9593-6_18.
This chapter provides methods for the propagation, purification, and titration of vaccinia virus (VACV) and the highly attenuated strain-modified vaccinia Ankara (MVA). Additionally, we provide information on VACV recombinants we have used for intravital imaging with multiphoton excitation.
本章提供了痘苗病毒（VACV）和高度减毒株——安卡拉痘苗病毒（MVA）的繁殖、纯化和滴定方法。此外，我们还提供了有关我们用于多光子激发活体成像的VACV重组体的信息。
