Cotter Catherine A, Earl Patricia L, Wyatt Linda S, Moss Bernard
National Institutes of Allergy and Infectious Diseases, Bethesda, Maryland.
Curr Protoc Microbiol. 2015 Nov 3;39:14A.3.1-14A.3.18. doi: 10.1002/9780471729259.mc14a03s39.
The culturing of cell lines used with vaccinia virus, both as monolayer and in suspension, is described. The preparation of chick embryo fibroblasts (CEF) is presented for use in the production of the highly attenuated and host range-restricted modified vaccinia virus Ankara (MVA) strain of vaccinia virus. Protocols for the preparation, titration, and trypsinization of vaccinia virus stocks, as well as viral DNA preparation and virus purification methods are also included.
本文描述了用于痘苗病毒的细胞系在单层培养和悬浮培养中的培养方法。介绍了鸡胚成纤维细胞（CEF）的制备方法，用于生产高度减毒且宿主范围受限的痘苗病毒安卡拉改良株（MVA）。还包括痘苗病毒储备液的制备、滴定和胰蛋白酶消化方案，以及病毒DNA制备和病毒纯化方法。
