细胞培养物和痘苗病毒储备液的制备。
Preparation of cell cultures and vaccinia virus stocks.
作者信息
Earl P L, Cooper N, Wyatt L S, Moss B, Carroll M W
机构信息
National Institutes of Allergy and Infectious Diseases, Bethesda, Maryland, USA.
出版信息
Curr Protoc Protein Sci. 2001 May;Chapter 5:Unit5.12. doi: 10.1002/0471140864.ps0512s13.
Abstract
This unit describes the maintenance of cell lines used with vaccinia virus, both in monolayer cultures and in suspension. The suspended cell culture is then used in the preparation of vaccinia virus stocks. The preparation of chick embryo fibroblasts (CEF) is also presented for use in the production of the highly attenuated and host range-restricted modified vaccinia virus Ankara (MVA) strain of vaccinia virus. Additionally, support protocols are presented for the titration of standard and MVA vaccinia virus stocks.
摘要
本单元介绍了痘苗病毒所用细胞系在单层培养和悬浮培养中的维持方法。然后，悬浮细胞培养用于痘苗病毒储备液的制备。还介绍了鸡胚成纤维细胞（CEF）的制备方法，用于生产高度减毒且宿主范围受限的痘苗病毒安卡拉（MVA）株。此外，还提供了标准痘苗病毒储备液和MVA痘苗病毒储备液滴定的支持方案。
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