College of First Clinical Medicine, Shandong University of Traditional Chinese Medicine, Jinan, Shandong, PR China.
Department of Oncology, Weifang Traditional Chinese Hospital, Weifang, Shandong, PR China.
J Cell Biochem. 2019 Nov;120(11):18956-18966. doi: 10.1002/jcb.29216. Epub 2019 Jun 26.
With the development in research, the importance of microRNAs (miRNAs) in the occurrence and metastasis, and prognosis of lung squamous cell carcinoma (LUSC) have received extensive attention. This study aims to identify new biomarkers and pivotal genes associated with LUSC prognosis through bioinformatics.
We downloaded miRNA and messenger RNA samples related to LUSC from The Cancer Genome Atlas (TCGA) database. Following initial data screening and preprocessing, we utilized the R platform and a range of analysis tools (miRDB, TargetScanHuman7.2, DAVID, and Cytoscape_v3.5.1) to analyze the TCGA data and identify highly specific and sensitive biomarkers.
We finally identified 12 miRNAs and six central genes closely related to the overall survival of patients with LUSC. We found that high expression of 7 miRNAs (miR-301b, miR-383, miR-512, miR-515, miR-525, miR-577, and miR-5682) and low expression of five miRNAs (miR-448, miR-486, miR-4732,miR-4732, miR-516, and miR-1911) can significantly improve the overall survival rate of patients with LUSC. The six central genes DLGAP5, CENPA, CHEK1, LRRK2, CALB1, and TOP2A are directly or indirectly involved in the formation and metastasis of LUSC and could, therefore, be considered as target genes for drug therapy.
With the development in research, the importance of miRNAs in the occurrence and metastasis and prognosis of LUSC have received extensive attention. This study aims to identify new biomarkers and pivotal genes associated with LUSC prognosis through bioinformatics.
随着研究的发展，微小 RNA（miRNA）在肺鳞状细胞癌（LUSC）的发生、转移和预后中的重要性受到了广泛关注。本研究旨在通过生物信息学方法鉴定与 LUSC 预后相关的新生物标志物和关键基因。
我们从癌症基因组图谱（TCGA）数据库下载了与 LUSC 相关的 miRNA 和信使 RNA 样本。经过初步的数据筛选和预处理，我们利用 R 平台和一系列分析工具（miRDB、TargetScanHuman7.2、DAVID 和 Cytoscape_v3.5.1）对 TCGA 数据进行分析，以鉴定高度特异和敏感的生物标志物。
我们最终确定了 12 个 miRNA 和 6 个与 LUSC 患者总生存率密切相关的核心基因。我们发现，7 个 miRNA（miR-301b、miR-383、miR-512、miR-515、miR-525、miR-577 和 miR-5682）的高表达和 5 个 miRNA（miR-448、miR-486、miR-4732、miR-4732、miR-516 和 miR-1911）的低表达可以显著提高 LUSC 患者的总生存率。这六个核心基因 DLGAP5、CENPA、CHEK1、LRRK2、CALB1 和 TOP2A 直接或间接参与了 LUSC 的形成和转移，因此可以被认为是药物治疗的靶基因。
随着研究的发展，miRNA 在 LUSC 的发生、转移和预后中的重要性受到了广泛关注。本研究旨在通过生物信息学方法鉴定与 LUSC 预后相关的新生物标志物和关键基因。
