Jiangsu Key Laboratory of Molecular Biology for Skin Diseases and STIs, Institute of Dermatology, Chinese Academy of Medical Sciences and Peking Union Medical College, Nanjing, China.
Jiangsu Key Laboratory of Molecular Biology for Skin Diseases and STIs, Institute of Dermatology, Chinese Academy of Medical Sciences and Peking Union Medical College, Nanjing, China,
Dermatology. 2019;235(5):418-425. doi: 10.1159/000500401. Epub 2019 Jun 26.
Condylomata acuminata are benign anogenital warts caused by human papillomavirus (HPV) infection with a high recurrence rate. Autophagy plays an important role in maintaining internal environmental stability. However, the role of autophagy regulation in the anogenital warts caused by HPV infection remains unknown.
A multigroup case-control study was designed to identify the autophagy gene fingerprint involved in anogenital warts arising from infections with different HPV genotypes.
Human autophagy PCR arrays were performed on the initial 18 participants grouped by their different HPV genotypes for gene expression-profiling analysis. The negative control was skin samples collected during plastic surgery on the chest from a group of individuals who showed none of the clinical symptoms or evidence of HPV infection. Real-time polymerase chain reaction (qPCR) was performed to validate the microarray results in another 24 individuals.
Out of 84 genes involved in autophagy, different autophagic responses were found among the 29 genes that encode autophagy machinery components, and expression levels of 13 of these genes were downregulated. Finally, we verified that the expression levels of 2 key genes that participate in the formation of autophagosomes, ATG3 and -BECLIN1, were downregulated in the HPV infection groups independently of genotype compared with the control group.
These findings showed that HPV infection downregulated the expression of ATGs in CA. Additionally, there were no differences in the expression of ATGs between the different HPV genotype infection groups. This study provided new insights into the autophagic response to HPV infection.
尖锐湿疣是由人乳头瘤病毒（HPV）感染引起的良性肛门生殖器疣，具有较高的复发率。自噬在维持内部环境稳定方面起着重要作用。然而，HPV 感染引起的肛门生殖器疣中自噬的调节作用尚不清楚。
设计了一项多组病例对照研究，以确定不同 HPV 基因型感染引起的肛门生殖器疣中涉及的自噬基因特征。
对最初的 18 名参与者按其不同的 HPV 基因型分组进行人类自噬 PCR 阵列，进行基因表达谱分析。阴性对照为从一组无任何临床症状或 HPV 感染证据的个体胸部整形手术采集的皮肤样本。对另外 24 名个体进行实时聚合酶链反应（qPCR）验证微阵列结果。
在涉及自噬的 84 个基因中，在编码自噬机制成分的 29 个基因中发现了不同的自噬反应，其中 13 个基因的表达水平下调。最后，我们验证了参与自噬体形成的 2 个关键基因 ATG3 和 -BECLIN1 的表达水平在 HPV 感染组中独立于基因型与对照组相比下调。
这些发现表明 HPV 感染下调了 CA 中 ATG 的表达。此外，不同 HPV 基因型感染组中 ATG 的表达无差异。本研究为 HPV 感染的自噬反应提供了新的见解。
