Centre for Women's Infectious Disease, Royal Women's Hospital, Parkville, Victoria, Australia
Molecular Microbiology Research Group, Murdoch Children's Research Institute, Parkville, Victoria, Australia.
J Clin Microbiol. 2019 Aug 26;57(9). doi: 10.1128/JCM.00344-19. Print 2019 Sep.
is a common sexually transmitted infection with a propensity to acquire resistance to commonly used antimicrobial therapies. Bacterial load has been linked to patient symptoms and the success of treatment. In this study, we demonstrate methodology to estimate load from routine diagnostic assays using the ResistancePlus MG test (SpeeDx Pty Ltd., Australia). The method gave comparable quantitation to an -specific 16S rRNA quantitative PCR (qPCR; Spearman = 0.94) for the samples analyzed ( = 499, including urine and swab types as detailed below) and was, therefore, employed to analyze typical load levels for samples in a diagnostic laboratory (total of 1,012 tests). When stratified by sample type, female urine (median, 826 genomes/ml) had the lowest load. This was significantly lower than median loads for all other sample types (male urine [6.91 × 10 genomes/ml], anal swabs [5.50 × 10], cervical swabs [8.15 × 10], endocervical swabs [3.97 × 10], and vaginal swabs [6.95 × 10]) ( < 0.0001). There were no significant differences in load estimates between the other sample types. Reproducibility of load estimates conducted on the same samples was high (r > 0.85). In conclusion, this methodology to provide load estimates for can be easily integrated into routine diagnostic laboratory workflow. Given the association between organism load, symptoms, and treatment success, load assessment has future diagnostic potential.
是一种常见的性传播感染，容易产生对常用抗菌治疗的耐药性。细菌负荷与患者症状和治疗效果有关。在这项研究中，我们展示了一种使用 ResistancePlus MG 测试（SpeeDx Pty Ltd.，澳大利亚）从常规诊断检测中估计负荷的方法。该方法与特定的 16S rRNA 定量 PCR（qPCR；Spearman = 0.94）对分析样本（ = 499，包括下文详述的尿液和拭子类型）进行了相当的定量，因此被用于分析诊断实验室中典型的样本负荷水平（总共 1012 次测试）。按样本类型分层时，女性尿液（中位数，826 个基因组/ml）的负荷最低。这显著低于所有其他样本类型的中位数负荷（男性尿液[6.91 × 10 个基因组/ml]、肛门拭子[5.50 × 10]、宫颈拭子[8.15 × 10]、阴道拭子[6.95 × 10]）（ < 0.0001）。其他样本类型之间的负荷估计值没有显著差异。对相同样本进行的负荷估计的重现性很高（r > 0.85）。总之，这种提供 负荷估计的方法可以很容易地整合到常规诊断实验室工作流程中。鉴于生物体负荷与症状和治疗成功之间的关联，负荷评估具有未来的诊断潜力。
