Institute for Bioengineering of Catalonia (IBEC), The Barcelona Institute of Science and Technology (BIST), Baldiri I Reixac, 10-12, 08028, Barcelona, Spain.
Lab Chip. 2019 Aug 7;19(15):2568-2580. doi: 10.1039/c9lc00285e. Epub 2019 Jun 27.
Despite the increasing number of organs-on-a-chip that have been developed in the past decade, limited efforts have been made to integrate a sensing system for in situ continual measurements of biomarkers from three-dimensional (3D) tissues. Here, we present a custom-made integrated platform for muscle cell stimulation under fluidic conditions connected with a multiplexed high-sensitivity electrochemical sensing system for in situ monitoring. To demonstrate this, we use our system to measure the release levels and release time of interleukin 6 and tumor necrosis factor alpha in vitro by 3D muscle microtissue under electrical and biological stimulations. Our experimental design has enabled us to perform multiple time point measurements using functionalized screen-printed gold electrodes with sensitivity in the ng mL range. This affordable setup is uniquely suited for monitoring factors released by 3D single cell types upon external stimulation for metabolic studies.
尽管在过去十年中已经开发出越来越多的器官芯片，但在将传感系统集成到原位连续测量三维（3D）组织生物标志物方面，所做的努力有限。在这里，我们提出了一种定制的集成平台，用于在流体条件下刺激肌肉细胞，并与用于原位监测的多路高灵敏度电化学生物传感系统相连。为了证明这一点，我们使用该系统通过电和生物刺激来测量体外 3D 肌肉微组织中白细胞介素 6 和肿瘤坏死因子 α 的释放水平和释放时间。我们的实验设计使我们能够使用功能化的丝网印刷金电极进行多次时间点测量，其灵敏度在纳克毫升范围内。这种经济实惠的设置非常适合监测 3D 单细胞类型在外部刺激下释放的代谢研究因素。
