Department of Chemistry, Faculty of Science, Chulalongkorn University, 254 Phayathai Road, Pathumwan, Bangkok, 10330, Thailand.
Electrochemistry and Optical Spectroscopy Center of Excellence (EOSCE), Department of Chemistry, Faculty of Science, Chulalongkorn University, Pathumwan, Bangkok, 10130, Thailand.
Mikrochim Acta. 2019 Jun 26;186(7):472. doi: 10.1007/s00604-019-3559-6.
A paper-based electrochemical sensor is described that is based on the use of thiol-terminated poly(2-methacryloyloxyethyl phosphorylcholine) (PMPC-SH) that was self-assembled on a gold nanoparticle-modified screen-printed electrode (SPE). The SPE sensor was used for label-free detection of C-reactive protein (CRP). Gold nanoparticles (AuNPs) were first electrodeposited on the SPCE, followed by the self-assembly of PMPC-SH on gold. The electrochemical response of the modified SPE to CRP was measured by differential pulse voltammetry (DPV). If the CRP on the paper device is contacted with Ca (II) ions, the current (measured by using hexacyanoferrate as the electrochemical probe) decreases. The signal drops in the 5 to 5000 ng·mL CRP concentration range, and the lower detection limit (at 3 SD/slope) is 1.6 ng·mL. The use of a PMPC-modified surface also reduces the nonspecific adsorption of proteins. The sensor is not interfered by bilirubin, myoglobin and albumin. It was successfully applied to CRP detection in certified human serum. This sensor is applicable as an attractive protocol for an inexpensive, highly sensitive, and disposable material for electrochemical detection of CRP. Graphical abstract Schematic presentation of highly sensitive and disposable paper-based electrochemical sensor using thiol-terminated poly(2-methacryloyloxyethyl phosphorylcholine) in the presence of Ca for the label-free C-reactive protein detection. The current was measured by differential pulse voltammetry.
一种基于金纳米粒子修饰的丝网印刷电极（SPE）上自组装的巯基封端聚（2-甲基丙烯酰氧乙基磷酸胆碱）（PMPC-SH）的纸基电化学传感器被描述。该 SPE 传感器用于无标记检测 C 反应蛋白（CRP）。首先在 SPCE 上电沉积金纳米粒子（AuNPs），然后在金上自组装 PMPC-SH。通过差分脉冲伏安法（DPV）测量修饰后的 SPE 对 CRP 的电化学响应。如果纸上装置上的 CRP 与 Ca（II）离子接触，电流（用六氰合铁酸盐作为电化学探针测量）会下降。信号在 5 至 5000ng·mL CRP 浓度范围内下降，检测下限（在 3 SD/斜率处）为 1.6ng·mL。使用 PMPC 修饰表面还可以减少蛋白质的非特异性吸附。该传感器不受胆红素、肌红蛋白和白蛋白的干扰。它成功地应用于认证的人血清中 CRP 的检测。该传感器适用于 CRP 检测的有吸引力的方案，因为它使用了廉价、高灵敏度和一次性的电化学检测 CRP 的材料。 图表摘要 用硫醇封端聚（2-甲基丙烯酰氧乙基磷酸胆碱）在 Ca 的存在下用于无标记 C 反应蛋白检测的灵敏且一次性的基于纸的电化学传感器的示意性表示。通过差分脉冲伏安法测量电流。
