Department of Chemistry , Johns Hopkins University , 3400 N. Charles Street , Baltimore , Maryland 21218 , United States.
J Am Chem Soc. 2019 Jul 3;141(26):10154-10158. doi: 10.1021/jacs.9b03686. Epub 2019 Jun 25.
Electron deficient "holes" migrate over long distances through the π-system in free DNA. Hole transfer efficiency (HTE) is strongly dependent on sequence and π-stacking. However, there is no consensus regarding the effects of nucleosome core particle (NCP) environment on hole migration. We quantitatively determined HTE in free DNA and NCPs by independently generating holes at specific positions in DNA. The relative HTE varied widely with respect to position within the NCP and proximity to tyrosine, which suppresses hole transfer. These data indicate that hole transfer in chromatin will be affected by the DNA sequence and its position with respect to histone proteins within NCPs.
缺电子的“空穴”通过自由 DNA 中的π系统长距离迁移。空穴转移效率(HTE)强烈依赖于序列和π-堆积。然而,核小体核心颗粒(NCP)环境对空穴迁移的影响尚无共识。我们通过在 DNA 的特定位置独立产生空穴来定量确定自由 DNA 和 NCP 中的 HTE。相对于 NCP 内的位置和酪氨酸的接近程度,相对 HTE 变化很大,酪氨酸会抑制空穴转移。这些数据表明,染色质中的空穴转移将受到 DNA 序列及其相对于 NCP 中组蛋白的位置的影响。