Division of Chemistry and Chemical Engineering , California Institute of Technology , Pasadena , California 91125 , United States.
ACS Chem Biol. 2018 Jul 20;13(7):1799-1809. doi: 10.1021/acschembio.8b00347. Epub 2018 Jun 1.
DNA charge transport chemistry involves the migration of charge over long molecular distances through the aromatic base pair stack within the DNA helix. This migration depends upon the intimate coupling of bases stacked one with another, and hence any perturbation in that stacking, through base modifications or protein binding, can be sensed electrically. In this review, we describe the many ways DNA charge transport chemistry has been utilized to sense changes in DNA, including the presence of lesions, mismatches, DNA-binding proteins, protein activity, and even reactions under weak magnetic fields. Charge transport chemistry is remarkable in its ability to sense the integrity of DNA.
DNA 电荷输运化学涉及电荷通过 DNA 螺旋中的芳香碱基对堆叠在长分子距离上的迁移。这种迁移取决于碱基彼此堆叠的紧密耦合，因此任何堆叠的扰动，通过碱基修饰或蛋白质结合，都可以被电感应到。在这篇综述中，我们描述了 DNA 电荷输运化学被用来感应 DNA 变化的许多方法，包括损伤、错配、DNA 结合蛋白、蛋白质活性，甚至在弱磁场下的反应。电荷输运化学在其感知 DNA 完整性的能力方面是卓越的。
