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一种来自A60的新型蛋白激发子PeBL2可诱导烟草植株对……产生系统抗性。 （原文中“against”后面内容缺失）

A Novel Protein Elicitor PeBL2, from A60, Induces Systemic Resistance against in Tobacco Plant.

作者信息

Jatoi Ghulam Hussain, Lihua Guo, Xiufen Yang, Gadhi Muswar Ali, Keerio Azhar Uddin, Abdulle Yusuf Ali, Qiu Dewen

机构信息

State Key Laboratory of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, No. 12 Zhongguancun South Street, Beijing 100081, China.

Department of Plant Pathology Sindh Agriculture University Tandojam, Sindh, Pakistan.

出版信息

Plant Pathol J. 2019 Jun;35(3):208-218. doi: 10.5423/PPJ.OA.11.2018.0276. Epub 2019 Jun 1.

DOI:10.5423/PPJ.OA.11.2018.0276
PMID:31244567
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6586191/
Abstract

Here, we reported a novel secreted protein elicitor PeBL2 from A60, which can induce hypersensitive response in tobacco (). The ion-exchange chromatography, high-performance liquid chromatography (HPLC) and mass spectrometry were performed for identification of protein elicitor. The 471 bp PeBL2 gene produces a 17.22 kDa protein with 156 amino acids containing an 84-residue signal peptide. Consistent with endogenous protein, the recombinant protein expressed in induced the typical hypersensitive response (HR) and necrosis in tobacco leaves. Additionally, PeBL2 also triggered early defensive response of generation of reactive oxygen species (HO and O ) and systemic resistance against of . Our findings shed new light on a novel strategy for biocontrol using A60.

摘要

在此，我们报道了一种来自A60的新型分泌蛋白激发子PeBL2，其可在烟草中诱导过敏反应。采用离子交换色谱、高效液相色谱（HPLC）和质谱对蛋白激发子进行鉴定。471 bp的PeBL2基因产生一种17.22 kDa的蛋白质，含156个氨基酸，其中有一个84个残基的信号肽。与内源蛋白一致，在[具体表达系统未明确]中表达的重组蛋白在烟草叶片中诱导了典型的过敏反应（HR）和坏死。此外，PeBL2还引发了活性氧（HO和O）产生的早期防御反应以及对[具体病原体未明确]的系统抗性。我们的研究结果为利用A60进行生物防治的新策略提供了新线索。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/85db/6586191/e168df8668ff/ppj-35-208f7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/85db/6586191/72db61d4572a/ppj-35-208f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/85db/6586191/3765c64d6c2c/ppj-35-208f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/85db/6586191/cced6effc157/ppj-35-208f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/85db/6586191/9427e1ef210a/ppj-35-208f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/85db/6586191/a47a9596433c/ppj-35-208f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/85db/6586191/42c1a5d1c195/ppj-35-208f6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/85db/6586191/e168df8668ff/ppj-35-208f7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/85db/6586191/72db61d4572a/ppj-35-208f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/85db/6586191/3765c64d6c2c/ppj-35-208f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/85db/6586191/cced6effc157/ppj-35-208f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/85db/6586191/9427e1ef210a/ppj-35-208f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/85db/6586191/a47a9596433c/ppj-35-208f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/85db/6586191/42c1a5d1c195/ppj-35-208f6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/85db/6586191/e168df8668ff/ppj-35-208f7.jpg

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