Department of Chemical Engineering, National Institute of Technology Rourkela, Rourkela, Odisha, 769 008. India.
J Environ Manage. 2019 Oct 1;247:196-204. doi: 10.1016/j.jenvman.2019.06.042. Epub 2019 Jun 24.
This study reports a novel method of using algae biomass as a source of lipid and various other co-products. Solvent-based extraction techniques could yield a number of products simultaneously. Further, the study focuses on all possible characterization and utilization of the three layers obtained from chloroform-methanol extraction of lipids. The lipid from the chloroform layer was transesterified for Fatty acid methyl esters (FAME) production. The fatty acid methyl esters derived by oleic acid, palmitic acid, linoleic acid and phytol were majorly analysed by GC-MS. The methanol layer was analysed with HPLC and stachyose, maltotriose, glucose, fructose, acetic acid, butyric acid, DMSO, glycerol were identified. The cell debris was further physically activated, and physiochemical properties of raw algae, residual algae and algae bio-char were compared. Spectrum peaks of FTIR study identified many alkyl‒halide stretches. Similarly, EDX analysed the presence of carbon, nitrogen, oxygen, potassium, chlorine, calcium, iron, magnesium and phosphorus. The SEM reveals that residual algae was comparatively crystalline and hence could not be utilized directly as an adsorbent. Therefore, further physical treatment was applied, and methylene blue dye adsorption study was also conducted to know the time and capacity of biochar as an adsorbent. However, organic and mineral enriched biomass could be used directly as fertilizer for agricultural purposes.
本研究报告了一种利用藻类生物质作为脂质和其他各种副产品来源的新方法。基于溶剂的提取技术可以同时产生多种产品。此外，本研究还重点关注了从氯仿-甲醇提取脂质中获得的三层物质的所有可能的特性和利用。从氯仿层提取的脂质进行酯交换以生产脂肪酸甲酯（FAME）。通过气相色谱-质谱（GC-MS）对油酸、棕榈酸、亚油酸和植醇衍生的脂肪酸甲酯进行了主要分析。通过高效液相色谱（HPLC）对甲醇层进行了分析，鉴定出了棉子糖、麦芽三糖、葡萄糖、果糖、乙酸、丁酸、DMSO、甘油。然后对细胞残渣进行物理活化，并比较了原藻、残留藻和藻类生物炭的理化性质。傅里叶变换红外（FTIR）研究的谱峰识别出了许多烷基卤化物伸展。同样，能量色散 X 射线分析（EDX）分析了碳、氮、氧、钾、氯、钙、铁、镁和磷的存在。扫描电子显微镜（SEM）显示，残留藻类比较结晶，因此不能直接用作吸附剂。因此，进一步进行了物理处理，并进行了亚甲基蓝染料吸附研究，以了解生物炭作为吸附剂的时间和容量。然而，富含有机和矿物质的生物质可以直接用作农业肥料。
