Key Laboratory of High Magnetic Field and Ion Beam Physical Biology, Institute of Technical Biology and Agriculture Engineering, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei 230031, China; University of Science and Technology of China, Hefei 230026, China.
Key Laboratory of High Magnetic Field and Ion Beam Physical Biology, Institute of Technical Biology and Agriculture Engineering, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei 230031, China; University of Science and Technology of China, Hefei 230026, China.
Spectrochim Acta A Mol Biomol Spectrosc. 2019 Dec 5;223:117282. doi: 10.1016/j.saa.2019.117282. Epub 2019 Jun 17.
A sensitive approach based on surface enhanced Raman spectroscopy (SERS) has been developed to evaluate the radiation caused biological injury. To achieve the effective SERS substrate, canonical anodic aluminum oxide (AAO) templates with regular array of nanotips were fabricated, and by plasma sputtering the gold nanoparticles (Au-NPs) were distributed on the nanotips to form the Au-NPs array with plenty of hotspots. The SERS substrates were utilized to examine the serum samples taken from the mice with the treatment of total body irradiation (TBI) of X-ray. The impact of TBI on the mice was analyzed and it was found that the SERS peak intensity at 532 cm increased as a function of duration or dose of TBI. We confirmed that this Raman signature belongs to the myoglobin as a biomarker for the muscle damage due to the radiation caused injury. Furthermore, we also tested several blood and urine specimen of cancer patients who received radiotherapy. The results showed that our approach to some extent could distinguish the bio-samples from normal, X-ray treated and untreated individuals. Therefore, the proposed methodology may have the potential for prompt prognosis of radiation injury at early stage.
一种基于表面增强拉曼光谱（SERS）的灵敏方法已被开发出来，用于评估辐射引起的生物损伤。为了获得有效的 SERS 基底，我们制备了具有规则纳米尖阵列的典型阳极氧化铝（AAO）模板，并通过等离子体溅射将金纳米颗粒（Au-NPs）分布在纳米尖上，从而在纳米尖上形成具有大量热点的 Au-NPs 阵列。我们利用 SERS 基底检测了接受 X 射线全身照射（TBI）治疗的小鼠的血清样本。分析了 TBI 对小鼠的影响，结果发现，随着 TBI 时间或剂量的增加，SERS 谱在 532cm 处的峰值强度增加。我们证实，这种拉曼特征属于肌红蛋白，是由于辐射引起的损伤导致肌肉损伤的生物标志物。此外，我们还测试了接受放疗的癌症患者的一些血液和尿液样本。结果表明，我们的方法在某种程度上可以区分正常、X 射线处理和未处理的生物样本。因此，该方法可能具有在早期阶段快速预测辐射损伤的潜力。
