State Key Laboratory of Agricultural Microbiology, College of Food Science and Technology, College of Science , Huazhong Agricultural University , Wuhan , Hubei 430070 , People's Republic of China.
Hubei Provincial Engineering and Technology Research Center for Food Quality and Safety Test , Hubei Provincial Institute for Food Supervision and Test , Wuhan , Hubei 430075 , People's Republic of China.
Anal Chem. 2019 Mar 19;91(6):3885-3892. doi: 10.1021/acs.analchem.8b04622. Epub 2019 Mar 6.
Surface-enhanced Raman spectroscopy (SERS) is a promising analytical tool, but simultaneous detection of multiple targets using SERS remains a challenge. Herein, a cauliflower-inspired 3D SERS substrate with intense hot spots was prepared through sputtering Au nanoparticles (Au NPs) on the surface of polydimethylsiloxane coated anodic aluminum oxide (PDMS@AAO) complex substrate. As a result, the cauliflower-inspired 3D SERS substrate achieved the highest SERS activities at a sputtering time of 8 min. Under the optimal conditions, this SERS substrate possessed a low detection limit of 10 M, excellent enhancement uniformity (relative standard deviation, RSD = 4.57%) and high enhancement factor (2.2 × 10) for 4-mercaptobenzoic acid (4-MBA). Furthermore, the results of Raman showed that the 3D-Nanocauliflower SERS substrates could realize the simultaneous label-free detection for three mycotoxins (aflatoxin B, deoxynivalenol, and zearalenone) in maize for the first time. It behaved good linear relationship between the concentrations and Raman intensities of aflatoxin B, zearalenone, and deoxynivalenol. For the three mycotoxins, this method exhibited the limit of detection (LOD) of 1.8, 47.7, and 24.8 ng/mL (S/N = 3), respectively. The 3D-Nanocauliflower SERS substrates with dense hot spots presented remarkable SERS effect and activity, which could be act as a potential candidate for SERS substrate applied in the rapid and label-free detection.
表面增强拉曼光谱（SERS）是一种很有前途的分析工具，但同时检测多个目标仍然是一个挑战。在此，通过在涂有聚二甲基硅氧烷的阳极氧化铝（PDMS@AAO）复合基底表面溅射金纳米粒子（Au NPs），制备了具有密集热点的类菜花 3D SERS 基底。结果表明，在溅射时间为 8 分钟时，类菜花 3D SERS 基底具有最高的 SERS 活性。在最佳条件下，该 SERS 基底对 4-巯基苯甲酸（4-MBA）的检测限低至 10 M，增强均匀性好（相对标准偏差，RSD = 4.57%），增强因子高（2.2×10）。此外，拉曼结果表明，3D-纳米菜花 SERS 基底首次实现了玉米中三种真菌毒素（黄曲霉毒素 B、脱氧雪腐镰刀菌烯醇和玉米赤霉烯酮）的无标记同时检测。黄曲霉毒素 B、玉米赤霉烯酮和脱氧雪腐镰刀菌烯醇的浓度与拉曼强度之间呈良好的线性关系。对于这三种真菌毒素，该方法的检出限（LOD）分别为 1.8、47.7 和 24.8 ng/mL（S/N = 3）。具有密集热点的 3D-纳米菜花 SERS 基底表现出显著的 SERS 效应和活性，可作为快速无标记检测中 SERS 基底的潜在候选材料。
