Institute of Plant Nutritional Physiology and Molecular Biology, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
College of Resources and Environment, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
Int J Mol Sci. 2019 Jun 26;20(13):3129. doi: 10.3390/ijms20133129.
Magnesium (Mg) deficiency is one of the major constraining factors that limit the yield and quality of agricultural products. Uniform seedlings of the were irrigated with Mg deficient (0 mM MgSO) and Mg sufficient (1 mM MgSO) nutrient solutions for 16 weeks. CO assimilation, starch, soluble carbohydrates, TBARS content and HO production were measured. Transcriptomic analysis of leaves was performed by Illumina sequencing. Our results showed that Mg deficiency decreased CO assimilation, but increased starch, sucrose, TBARS content and HO production in leaves. A total of 4864 genes showed differential expression in response to Mg deficiency revealed by RNA-Seq and the transcriptomic data were further validated by real-time quantitative PCR (RT-qPCR). Gene ontology (GO) enrichment analysis indicated that the mechanisms underlying Mg deficiency tolerance in may be attributed to the following aspects: a) enhanced microtubule-based movement and cell cycle regulation; b) elevated signal transduction in response to biotic and abiotic stimuli; c) alteration of biological processes by tightly controlling phosphorylation especially protein phosphorylation; d) down-regulation of light harvesting and photosynthesis due to the accumulation of carbohydrates; e) up-regulation of cell wall remodeling and antioxidant system. Our results provide a comprehensive insight into the transcriptomic profile of key components involved in the Mg deficiency tolerance in and enrich our understanding of the molecular mechanisms by which plants adapted to a Mg deficient condition.
镁（Mg）缺乏是限制农产品产量和品质的主要限制因素之一。将均匀的 幼苗用缺镁（0 mM MgSO）和富镁（1 mM MgSO）营养液灌溉 16 周。测量 CO 同化、淀粉、可溶性碳水化合物、TBARS 含量和 HO 生成。通过 Illumina 测序对 叶片进行转录组分析。我们的结果表明，Mg 缺乏降低了 CO 的同化，但增加了 叶片中的淀粉、蔗糖、TBARS 含量和 HO 的产生。总共有 4864 个基因对 Mg 缺乏的响应表现出差异表达，这是通过 RNA-Seq 揭示的，转录组数据进一步通过实时定量 PCR（RT-qPCR）进行验证。基因本体（GO）富集分析表明， 在 Mg 缺乏耐受中的机制可能归因于以下几个方面：a）增强微管基于运动和细胞周期调节；b）提高对生物和非生物刺激的信号转导；c）通过严格控制磷酸化特别是蛋白质磷酸化来改变生物过程；d）由于碳水化合物的积累，光捕获和光合作用的下调；e）细胞壁重塑和抗氧化系统的上调。我们的研究结果为 中参与 Mg 缺乏耐受的关键成分的转录组图谱提供了全面的见解，并丰富了我们对植物适应 Mg 缺乏条件的分子机制的理解。
