Escuela de Odontología, Grupo biomateriales dentales, Universidad del Valle, Calle 13 No. 100-00, 76001 Cali, Colombia.
Laboratorio SIMERQO polímeros, Departamento de Química, Universidad del Valle, Calle 13 No. 100-00, 76001 Cali, Colombia.
Int J Mol Sci. 2019 Mar 29;20(7):1572. doi: 10.3390/ijms20071572.
In the last few years, graphene oxide (GO) has gained considerable importance in scaffold preparation for tissue engineering due to the presence of functional groups that allow the interaction between the extracellular matrix and the components of the cellular membrane. The interaction between GO and chitosan (CS) can not only improve the biomechanical properties of the scaffold but also generate a synergistic effect, facilitating tissue recovery. studies on GO are scarce; therefore, biocompatibility tests on CS-GO scaffolds and bone regeneration experiments on critical size defects were carried out on rats. Scaffolds made of CS, CS-GO 0.5%, and CS-GO 1% were prepared and implanted on rats cranial bones for three months. Scaffold samples were analyzed through histochemistry and scanning electron microscopy. The analysis performed showed reabsorption of the material by phagocytic activity and new bone formation. The CS-GO 0.5% formulation gave the best performance in bone regeneration, with excellent biocompatibility. These results show the potential of this compound for tissue regeneration opening and medical applications.
在过去的几年中，由于氧化石墨烯 (GO) 中存在允许细胞外基质与细胞膜成分相互作用的官能团，因此在组织工程支架制备方面引起了相当大的重视。GO 与壳聚糖 (CS) 的相互作用不仅可以改善支架的生物力学性能，还可以产生协同作用，促进组织恢复。目前关于 GO 的研究还很少；因此，对 CS-GO 支架进行了生物相容性测试，并在大鼠临界尺寸缺陷上进行了骨再生实验。制备了 CS、CS-GO 0.5%和 CS-GO 1%的支架，并将其植入大鼠颅骨中三个月。通过组织化学和扫描电子显微镜分析支架样本。进行的分析表明，材料被吞噬活性吸收并形成新骨。CS-GO 0.5%配方在骨再生方面表现最佳，具有极好的生物相容性。这些结果表明该化合物在组织再生和医学应用方面具有潜力。
