Laboratory of Applied Bioacoustics, Technical University of Catalonia, Barcelona TECH, 08800, Rambla exposició s/n, Vilanova i la Geltrú, Barcelona, Spain.
Proteomics Laboratory, Vall d'Hebron Institute of Oncology (VHIO), Edifici Collserola, 08035, Barcelona, Spain.
Sci Rep. 2019 Jun 27;9(1):9340. doi: 10.1038/s41598-019-45646-6.
Recent studies, both in laboratory and sea conditions, have demonstrated damage after sound exposure in the cephalopod statocyst sensory epithelium, which secretes endolymph protein. Here, the proteomic analysis of the endolymph was performed before and after sound exposure to assess the effects of exposure to low intensity, low frequency sounds on the statocyst endolymph of the Mediterranean common cuttlefish (Sepia officinalis), determining changes in the protein composition of the statocyst endolymph immediately and 24 h after sound exposure. Significant differences in protein expression were observed, especially 24 h after exposure. A total of 37 spots were significantly different in exposed specimens, 17 of which were mostly related to stress and cytoskeletal structure. Among the stress proteins eight spots corresponding to eight hemocyanin isoforms were under-expressed possible due to lower oxygen consumption. In addition, cytoskeletal proteins such as tubulin alpha chain and intermediate filament protein were also down-regulated after exposure. Thus, endolymph analysis in the context of acoustic stress allowed us to establish the effects at the proteome level and identify the proteins that are particularly sensitive to this type of trauma.
最近的实验室和海上研究表明，在头足类平衡囊感觉上皮中，分泌内淋巴蛋白的细胞在声音暴露后受到了损伤。在这里，我们对暴露前后的内淋巴进行了蛋白质组学分析，以评估低频、低强度声音暴露对地中海普通乌贼（Sepia officinalis）平衡囊内淋巴的影响，确定暴露后即刻和 24 小时内平衡囊内淋巴蛋白组成的变化。观察到蛋白质表达存在显著差异，尤其是暴露后 24 小时。暴露组的 37 个斑点差异显著，其中 17 个斑点主要与应激和细胞骨架结构有关。在应激蛋白中，有 8 个斑点对应 8 种血蓝蛋白同工型，由于耗氧量降低，这些同工型表达水平下调。此外，暴露后细胞骨架蛋白如微管蛋白α链和中间丝蛋白也下调。因此，在声应激背景下对内淋巴进行分析，使我们能够在蛋白质组水平上确定影响，并确定对这种类型的创伤特别敏感的蛋白质。
