Haba T, Takagi H, Nagura H
Department of Surgery II, Nagoya University School of Medicine, Japan.
Gastroenterol Jpn. 1991 Oct;26(5):593-602. doi: 10.1007/BF02781675.
The effect of Cyclosporin (CS) on the gut-associated lymphoid tissue (GALT) of inbred WKA rats was immunohistochemically studied by the immunoperoxidase method. CS in olive oil was orally administered daily for seven days at the dose of 25, 50 and 100 mg/kg/day. On days 1, 4 and 7 of CS administration, rats were sacrificed under ether anesthesia. By CS administration, the decrease and disappearance of Ia antigen expression were dose-dependently recognized on the epithelial cells and the overlaying cells of Peyer's patches. The reduced cellular population and Ia expression of lymphocytes of the follicle and the disappearance of the germinal center also occurred, and Ia-positive dendritic non-lymphoid cells in Peyer's patches and endothelial cells of capillaries in the lamina propria disappeared on day 7 of CS administration. In addition, a temporary increase of W3/25-positive cells and the appearance of Ia-positive intra-epithelial cells were observed on day 4. Simultaneously, interleukin 2 receptor (IL-2R)-positive cells gradually decreased on day 4, and almost disappeared on day 7. From these results, it is speculated that CS suppresses not only interleukin 2 (IL-2) production as previously reported, but also the expression of Ia antigens and IL-2R. It may well suppress the immune system at the point of recognition and presentation of alloantigen, and the proliferation and differentiation of B cells.
采用免疫过氧化物酶法，通过免疫组织化学研究了环孢素（CS）对近交系WKA大鼠肠道相关淋巴组织（GALT）的影响。将CS溶于橄榄油中，以25、50和100mg/kg/天的剂量每日口服给药7天。在CS给药的第1、4和7天，大鼠在乙醚麻醉下处死。通过CS给药，在派尔集合淋巴结的上皮细胞和覆盖细胞上，剂量依赖性地观察到Ia抗原表达的减少和消失。滤泡淋巴细胞数量减少、Ia表达降低以及生发中心消失也出现了，并且在CS给药第7天，派尔集合淋巴结中Ia阳性树突状非淋巴细胞和固有层毛细血管内皮细胞消失。此外，在第4天观察到W3/25阳性细胞暂时增加以及Ia阳性上皮内细胞出现。同时，白细胞介素2受体（IL-2R）阳性细胞在第4天逐渐减少，并在第7天几乎消失。从这些结果推测，CS不仅如先前报道的那样抑制白细胞介素2（IL-2）的产生，还抑制Ia抗原和IL-2R的表达。它很可能在同种异体抗原的识别和呈递以及B细胞的增殖和分化方面抑制免疫系统。
