Godlewska Renata, Klim Joanna, Dębski Janusz, Wyszyńska Agnieszka, Łasica Anna
Department of Bacterial Genetics, Institute of Microbiology, Faculty of Biology, University of Warsaw , Warsaw , Poland.
Mass Spectrometry Laboratory, Institute of Biochemistry and Biophysics, Polish Academy of Sciences , Warsaw , Poland.
Pol J Microbiol. 2019;68(2):255-261. doi: 10.33073/pjm-2019-027.
The proteomes of outer membrane vesicles (OMVs) secreted by 81-176 strain, which was exposed to oxygen or antibiotic stress (polymyxin B), were characterized. We also assessed the OMVs production and their content in two mutated strains - ∆ and ∆. OMVs production was significantly increased under the polymyxin B stress and remained unaltered in all other variants. Interestingly, the qualitative load of OMVs was constant regardless of the stress conditions or genetic background. However, certain proteins exhibited notable quantitative changes, ranging from 4-fold decrease to 10-fold increase. Up- or downregulated proteins (e.g. major outer membrane protein , iron ABC transporter, serine protease- , 60 kDa chaperonin-, enolase) represented various cell compartments (cytoplasm, periplasm, and membrane) and exhibited various functions; nevertheless, one common group was noted that consisted of components of flagellar apparatus, i.e., FlaA/B, FlgC/E, which were mostly upregulated. Some of these proteins are the putative substrates of DsbI protein. Further investigation of the regulation of OMVs composition and their role in virulence will allow a better understanding of the infectious process of . The proteomes of outer membrane vesicles (OMVs) secreted by 81–176 strain, which was exposed to oxygen or antibiotic stress (polymyxin B), were characterized. We also assessed the OMVs production and their content in two mutated strains – ∆ and ∆. OMVs production was significantly increased under the polymyxin B stress and remained unaltered in all other variants. Interestingly, the qualitative load of OMVs was constant regardless of the stress conditions or genetic background. However, certain proteins exhibited notable quantitative changes, ranging from 4-fold decrease to 10-fold increase. Up- or downregulated proteins (e.g. major outer membrane protein , iron ABC transporter, serine protease- , 60 kDa chaperonin-, enolase) represented various cell compartments (cytoplasm, periplasm, and membrane) and exhibited various functions; nevertheless, one common group was noted that consisted of components of flagellar apparatus, i.e., FlaA/B, FlgC/E, which were mostly upregulated. Some of these proteins are the putative substrates of DsbI protein. Further investigation of the regulation of OMVs composition and their role in virulence will allow a better understanding of the infectious process of .
对暴露于氧气或抗生素应激（多粘菌素B）下的81 - 176菌株分泌的外膜囊泡（OMV）的蛋白质组进行了表征。我们还评估了两种突变菌株——∆和∆中OMV的产生及其含量。在多粘菌素B应激下，OMV的产生显著增加，而在所有其他变体中保持不变。有趣的是，无论应激条件或遗传背景如何，OMV的定性负载都是恒定的。然而，某些蛋白质表现出显著的定量变化，范围从4倍减少到10倍增加。上调或下调的蛋白质（例如主要外膜蛋白、铁ABC转运蛋白、丝氨酸蛋白酶 - 、60 kDa伴侣蛋白 - 、烯醇酶）代表了不同的细胞区室（细胞质、周质和膜）并具有各种功能；然而，发现了一个共同的组，其由鞭毛装置的组件组成，即FlaA/B、FlgC/E，它们大多上调。这些蛋白质中的一些是DsbI蛋白的假定底物。对外膜囊泡组成的调节及其在毒力中的作用进行进一步研究将有助于更好地理解[具体病原体名称]的感染过程。对暴露于氧气或抗生素应激（多粘菌素B）下的81–176菌株分泌的外膜囊泡（OMV）的蛋白质组进行了表征。我们还评估了两种突变菌株——∆和∆中OMV的产生及其含量。在多粘菌素B应激下，OMV的产生显著增加，而在所有其他变体中保持不变。有趣的是，无论应激条件或遗传背景如何，OMV的定性负载都是恒定的。然而，某些蛋白质表现出显著的定量变化，范围从4倍减少到10倍增加。上调或下调的蛋白质（例如主要外膜蛋白、铁ABC转运蛋白、丝氨酸蛋白酶 - 、60 kDa伴侣蛋白 - 、烯醇酶）代表了不同的细胞区室（细胞质、周质和膜）并具有各种功能；然而，发现了一个共同的组，其由鞭毛装置的组件组成，即FlaA/B、FlgC/E，它们大多上调。这些蛋白质中的一些是DsbI蛋白的假定底物。对外膜囊泡组成的调节及其在毒力中的作用进行进一步研究将有助于更好地理解[具体病原体名称]的感染过程。
