Faculty of Chemistry, Jagiellonian University, Gronostajowa 2, 30-387 Kraków, Poland.
Faculty of Chemistry, Jagiellonian University, Gronostajowa 2, 30-387 Kraków, Poland; AGH University of Science and Technology, Faculty of Physics and Applied Computer Science, Mickiewicza 30, 30-059 Kraków, Poland.
Int J Biol Macromol. 2019 Sep 1;136:1196-1208. doi: 10.1016/j.ijbiomac.2019.06.184. Epub 2019 Jun 25.
Collagen, chitosan and hyaluronic acid based multicomponent injectable and in situ gellating biomimetic hybrid materials for bone tissue engineering applications were prepared in one-step procedure. The bioactive phase in the form of surface-modified silica particles was introduced to the solutions of biopolymers and simultaneously crosslinked with genipin both the biopolymer matrix and dispersed particles at 37 °C. The novel approach presented here involved the use of silica particles which surfaces were priory functionalized with amino groups. That modification makes possible the covalent attachment of silica particles to the polymeric hydrogel network on crosslinking with genipin. That methodology is especially important as it makes possible to obtain the hybrid materials (biopolymer-silica particles) in which the problems related to the potential phase separation of mineral particles, hindering their in vivo application can be eliminated. The hybrids of various compositions were obtained and their physicochemical and biological properties were determined. The in vitro experiments performed under simulated body fluid conditions revealed that the amino-functionalized silica particles covalently attached to the biopolymeric network are still bioactive. Finally, the in vitro cell culture studies shown that the materials developed are biocompatible as they supported MG-63 cells adhesion, proliferation as well as Alkaline phosphatase (ALP) expression.
胶原蛋白、壳聚糖和透明质酸基多组分可注射和原位凝胶化仿生杂化材料，用于骨组织工程应用，是通过一步法制备的。将生物活性相以表面改性的硅颗粒形式引入生物聚合物溶液中，并在 37°C 下同时用京尼平交联生物聚合物基质和分散的颗粒。这里提出的新方法涉及使用预先用氨基官能化的硅颗粒表面。这种修饰使得硅颗粒能够与京尼平交联时共价连接到聚合物水凝胶网络上。这种方法特别重要，因为它可以获得杂化材料（生物聚合物-硅颗粒），从而消除与矿物颗粒潜在相分离相关的问题，阻碍其在体内的应用。获得了各种组成的杂化物，并测定了它们的物理化学和生物学性质。在模拟体液条件下进行的体外实验表明，共价连接到生物聚合物网络上的氨基功能化硅颗粒仍然具有生物活性。最后，体外细胞培养研究表明，所开发的材料是生物相容的，因为它们支持 MG-63 细胞的黏附、增殖以及碱性磷酸酶（ALP）的表达。
